BMRB Entry 51067
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51067
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Murthy, Anastasia; Tang, Wai Shing; Jovic, Nina; Janke, Abigail; Seo, Da Hee; Perdikari, Theodora Myrto; Mittal, Jeetain; Fawzi, Nicolas. "Molecular interactions contributing to FUS SYGQ LC-RGG phase separation and co-partitioning with RNA polymerase II heptads" Nat. Struct. Mol. Biol. 28, 923-935 (2021).
PubMed: 34759379
Assembly members:
entity_1, polymer, 104 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTHMT
Entity Sequences (FASTA):
entity_1: SSGGGGGGGGGGNYGQDQSS
MSSGGGSGGGYGNQDQSGGG
GSGGYGQQDRGGRGRGGSGG
GGGGGGGGYNRSSGGYEPRG
RGGGRGGRGGMGGSDRGGFN
KFGG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 208 |
| 15N chemical shifts | 66 |
| 1H chemical shifts | 66 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FUS RGG1 | 1 |
Entities:
Entity 1, FUS RGG1 104 residues - Formula weight is not available
| 1 | SER | SER | GLY | GLY | GLY | GLY | GLY | GLY | GLY | GLY | ||||
| 2 | GLY | GLY | ASN | TYR | GLY | GLN | ASP | GLN | SER | SER | ||||
| 3 | MET | SER | SER | GLY | GLY | GLY | SER | GLY | GLY | GLY | ||||
| 4 | TYR | GLY | ASN | GLN | ASP | GLN | SER | GLY | GLY | GLY | ||||
| 5 | GLY | SER | GLY | GLY | TYR | GLY | GLN | GLN | ASP | ARG | ||||
| 6 | GLY | GLY | ARG | GLY | ARG | GLY | GLY | SER | GLY | GLY | ||||
| 7 | GLY | GLY | GLY | GLY | GLY | GLY | GLY | GLY | TYR | ASN | ||||
| 8 | ARG | SER | SER | GLY | GLY | TYR | GLU | PRO | ARG | GLY | ||||
| 9 | ARG | GLY | GLY | GLY | ARG | GLY | GLY | ARG | GLY | GLY | ||||
| 10 | MET | GLY | GLY | SER | ASP | ARG | GLY | GLY | PHE | ASN | ||||
| 11 | LYS | PHE | GLY | GLY |
Samples:
sample_1: FUS RGG1 (164-267), [U-99% 13C; U-99% 15N], 1.8 mM; MES 20 mM; sodium chloride 150 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 5.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRFAM-SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks