BMRB Entry 51062

Name: Backbone assignment of ATF4 transactivation domain
Published: 2021-09-18

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51062

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of ATF4 transactivation domain

Deposition date:

2021-08-23

Original release date:

2021-09-18

Authors:

Siang, Steven; Roche, Julien

Citation:

Citation: Siang, Steven; Underbakke, Eric; Roche, Julien. "Intricate coupling between the transactivation and basic-leucine zipper domains governs phosphorylation of transcription factor ATF4 by casein kinase 2" J. Biol. Chem. 298, 101633-101633 (2022).
PubMed: 35077711

Assembly members:

Assembly members:
entity_1, polymer, 275 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MTEMSFLSSEVLVGDLMSPF DQSGLGAEESLGLLDDYLEV AKHFKPHGFSSDKAKAGSSE WLAVDGLVSPSNNSKEDAFS GTDWMLEKMDLKEFDLDALL GIDDLETMPDDLLTTLDDTC DLFAPLVQETNKQPPQTVNP IGHLPESLTKPDQVAPFTFL QPLPLSPGVLSSTPDHSFSL ELGSEVDITEGDRKPDYTAY VAMIPQCIKEEDTPSDNDSG ICMSPESYLGSPQHSPSTRG SPNRSLPSPGVLCGSARPKP YDPPGEKMVAAKVKG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	687
15N chemical shifts	196
1H chemical shifts	196

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ATF4 transactivation domain	1

Entities:

Entity 1, ATF4 transactivation domain 275 residues - Formula weight is not available

1

MET

THR

GLU

MET

SER

PHE

LEU

SER

GLU

2

VAL

LEU

VAL

GLY

ASP

LEU

MET

SER

PRO

PHE

3

ASP

GLN

SER

GLY

LEU

GLY

ALA

GLU

SER

4

LEU

GLY

LEU

ASP

TYR

LEU

GLU

VAL

5

ALA

LYS

HIS

PHE

LYS

PRO

HIS

GLY

PHE

SER

6

SER

ASP

LYS

ALA

LYS

ALA

GLY

SER

GLU

7

TRP

LEU

ALA

VAL

ASP

GLY

LEU

VAL

SER

PRO

8

SER

ASN

SER

LYS

GLU

ASP

ALA

PHE

SER

9

GLY

THR

ASP

TRP

MET

LEU

GLU

LYS

MET

ASP

10

LEU

LYS

GLU

PHE

ASP

LEU

ASP

ALA

LEU

11

GLY

ILE

ASP

LEU

GLU

THR

MET

PRO

ASP

12

ASP

LEU

THR

LEU

ASP

THR

CYS

13

ASP

LEU

PHE

ALA

PRO

LEU

VAL

GLN

GLU

THR

14

ASN

LYS

GLN

PRO

GLN

THR

VAL

ASN

PRO

15

ILE

GLY

HIS

LEU

PRO

GLU

SER

LEU

THR

LYS

16

PRO

ASP

GLN

VAL

ALA

PRO

PHE

THR

PHE

LEU

17

GLN

PRO

LEU

PRO

LEU

SER

PRO

GLY

VAL

LEU

18

SER

THR

PRO

ASP

HIS

SER

PHE

SER

LEU

19

GLU

LEU

GLY

SER

GLU

VAL

ASP

ILE

THR

GLU

20

GLY

ASP

ARG

LYS

PRO

ASP

TYR

THR

ALA

TYR

21

VAL

ALA

MET

ILE

PRO

GLN

CYS

ILE

LYS

GLU

22

GLU

ASP

THR

PRO

SER

ASP

ASN

ASP

SER

GLY

23

ILE

CYS

MET

SER

PRO

GLU

SER

TYR

LEU

GLY

24

SER

PRO

GLN

HIS

SER

PRO

SER

THR

ARG

GLY

25

SER

PRO

ASN

ARG

SER

LEU

PRO

SER

PRO

GLY

26

VAL

LEU

CYS

GLY

SER

ALA

ARG

PRO

LYS

PRO

27

TYR

ASP

PRO

GLY

GLU

LYS

MET

VAL

ALA

28

ALA

LYS

VAL

LYS

GLY

Samples:

sample_1: ATF4 transactivation domain, [U-99% 15N], 175 uM; ATF4 transactivation domain, [U-13C; U-15N; U-2H], 175 uM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment, data analysis

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks