BMRB Entry 51059

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51059

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Title:
NMR assignments of the Fc fragment of human immunoglobulin G1 glycoprotein non-fucosylated, non-galactosylated (G0) glycoform
Deposition date:
2021-08-17
Original release date:
2022-03-11
Authors:
Yanaka, Saeko; Yagi, Hirokazu; Yogo, Rina; Onitsuka, Masayoshi; Kato, Koichi
Citation:

Citation: Yanaka, Saeko; Yagi, Hirokazu; Yogo, Rina; Onitsuka, Masayoshi; Kato, Koichi. "Glutamine-free mammalian expression of recombinant glycoproteins with uniform isotope labeling: an application for NMR analysis of pharmaceutically relevant Fc glycoforms of human immunoglobulin G1"  J. Biomol. NMR 76, 17-22 (2022).
PubMed: 34978013

Assembly members:

Assembly members:
entity_1, polymer, 222 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Chinese hamster ovary cells   Vector: Mammalian PowerExpress system vector

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: TCPPCPAPELLGGPSVFLFP PKPKDTLMISRTPEVTCVVV DVSHEDPEVKFNWYVDGVEV HNAKTKPREEQYNSTYRVVS VLTVLHQDWLNGKEYKCKVS NKALPAPIEKTISKAKGQPR EPQVYTLPPSRDELTKNQVS LTCLVKGFYPSDIAVEWESN GQPENNYKTTPPVLDSDGSF FLYSKLTVDKSRWQQGNVFS CSVMHEALHNHYTQKSLSLS PG

Data sets:
Data typeCount
15N chemical shifts180
1H chemical shifts180

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_1, chain 11
2entity_1, chain 21

Entities:

Entity 1, entity_1, chain 1 222 residues - Formula weight is not available

1   THRCYSPROPROCYSPROALAPROGLULEU
2   LEUGLYGLYPROSERVALPHELEUPHEPRO
3   PROLYSPROLYSASPTHRLEUMETILESER
4   ARGTHRPROGLUVALTHRCYSVALVALVAL
5   ASPVALSERHISGLUASPPROGLUVALLYS
6   PHEASNTRPTYRVALASPGLYVALGLUVAL
7   HISASNALALYSTHRLYSPROARGGLUGLU
8   GLNTYRASNSERTHRTYRARGVALVALSER
9   VALLEUTHRVALLEUHISGLNASPTRPLEU
10   ASNGLYLYSGLUTYRLYSCYSLYSVALSER
11   ASNLYSALALEUPROALAPROILEGLULYS
12   THRILESERLYSALALYSGLYGLNPROARG
13   GLUPROGLNVALTYRTHRLEUPROPROSER
14   ARGASPGLULEUTHRLYSASNGLNVALSER
15   LEUTHRCYSLEUVALLYSGLYPHETYRPRO
16   SERASPILEALAVALGLUTRPGLUSERASN
17   GLYGLNPROGLUASNASNTYRLYSTHRTHR
18   PROPROVALLEUASPSERASPGLYSERPHE
19   PHELEUTYRSERLYSLEUTHRVALASPLYS
20   SERARGTRPGLNGLNGLYASNVALPHESER
21   CYSSERVALMETHISGLUALALEUHISASN
22   HISTYRTHRGLNLYSSERLEUSERLEUSER
23   PROGLY

Samples:

sample_1: Fc fragment of human immunoglobulin G1 glycoprotein non-fucosylated, non-galactosylated (G0) glycoform, [U-100% 15N], 10 mg/mL; NaCl 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 315 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, NMRview - chemical shift assignment, collection

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks