BMRB Entry 51058

Name: RBM5 RRM1-ZnF1-RRM2 (C191G)
Published: 2023-06-01

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51058

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

RBM5 RRM1-ZnF1-RRM2 (C191G)

Deposition date:

2021-08-12

Original release date:

2023-06-01

Authors:

Soni, Komal; Jagtap, Pravin Kumar Ankush; Sattler, Michael

Citation:

Citation: Soni, Komal; Jagtap, Pravin Kumar Ankush; Martinez-Lumbreras, Santiago; Bonnal, Sophie; Geerlof, Arie; Stehle, Ralf; Simon, Bernd; Valcarcel, Juan; Sattler, Michael. "Structural basis for specific RNA recognition by the alternative splicing factor RBM5" Nat. Commun. 14, 4233-4233 (2023).
PubMed: 37454201

Assembly members:

Assembly members:
entity_1, polymer, 226 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Modified petM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMGERESKTIMLRGLPTTI TESDIREMMESFEGPQPADV RLMKRKTGVSRGFAFVEFYH LQDATSWMEANQKKLVIQGK HIAMHYSNPRPKFEDWLCNK CGLNNFRKRLKCFRCGADKF DSEQEVPPGTTESVQSVDYY CDTIILRNIAPHTVVDSIMT ALSPYASLAVNNIRLIKDKQ TQQNRGFAFVQLSSAMDASQ LLQILQSLHPPLKIDGKTIG VDFAKS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	186
1H chemical shifts	186

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RRM1-ZnF1-RRM2 (C191G)	1
2	Zn2+	2

Entities:

Entity 1, RRM1-ZnF1-RRM2 (C191G) 226 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

GLU

ARG

GLU

SER

LYS

THR

2

ILE

MET

LEU

ARG

GLY

LEU

PRO

THR

ILE

3

THR

GLU

SER

ASP

ILE

ARG

GLU

MET

GLU

4

SER

PHE

GLU

GLY

PRO

GLN

PRO

ALA

ASP

VAL

5

ARG

LEU

MET

LYS

ARG

LYS

THR

GLY

VAL

SER

6

ARG

GLY

PHE

ALA

PHE

VAL

GLU

PHE

TYR

HIS

7

LEU

GLN

ASP

ALA

THR

SER

TRP

MET

GLU

ALA

8

ASN

GLN

LYS

LEU

VAL

ILE

GLN

GLY

LYS

9

HIS

ILE

ALA

MET

HIS

TYR

SER

ASN

PRO

ARG

10

PRO

LYS

PHE

GLU

ASP

TRP

LEU

CYS

ASN

LYS

11

CYS

GLY

LEU

ASN

PHE

ARG

LYS

ARG

LEU

12

LYS

CYS

PHE

ARG

CYS

GLY

ALA

ASP

LYS

PHE

13

ASP

SER

GLU

GLN

GLU

VAL

PRO

GLY

THR

14

THR

GLU

SER

VAL

GLN

SER

VAL

ASP

TYR

15

CYS

ASP

THR

ILE

LEU

ARG

ASN

ILE

ALA

16

PRO

HIS

THR

VAL

ASP

SER

ILE

MET

THR

17

ALA

LEU

SER

PRO

TYR

ALA

SER

LEU

ALA

VAL

18

ASN

ILE

ARG

LEU

ILE

LYS

ASP

LYS

GLN

19

THR

GLN

ASN

ARG

GLY

PHE

ALA

PHE

VAL

20

GLN

LEU

SER

ALA

MET

ASP

ALA

SER

GLN

21

LEU

GLN

ILE

LEU

GLN

SER

LEU

HIS

PRO

22

PRO

LEU

LYS

ILE

ASP

GLY

LYS

THR

ILE

GLY

23

VAL

ASP

PHE

ALA

LYS

SER

Entity 2, Zn2+ - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: RBM5 RRM1-ZnF1-RRM2 (C191G), [U-100% 13C; U-100% 15N], 0.43 mM; D2O 10%; sodium chloride 400 mM; MES 20 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.4 M; pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 1H	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 950 MHz

UNP	P52756
AlphaFold	Q9UL24