BMRB Entry 51051

Name: S. cerevisiae SERF
Published: 2022-07-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51051

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

S. cerevisiae SERF

Deposition date:

2021-08-10

Original release date:

2022-07-14

Authors:

Liu, Yicong; Jin, Yangzhuoyue; Qiao, Yang

Citation:

Citation: Liu, Yicong; Wang, Chaozhe; Jin, Yangzhuoyue; Jiang, Guosheng; He, Lichun; Liu, Maili. "Backbone resonance assignments and dynamics of S. cerevisiae SERF" Biomol. NMR Assignments 16, 187-190 (2022).
PubMed: 35713792

Assembly members:

Assembly members:
entity_1, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MARGNQRDLARQKNLKKQKD MAKNQKKSGDPKKRMESDAE ILRQKQAAADARREAEKLEK LKAEKTRR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	191
15N chemical shifts	67
1H chemical shifts	66

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	S. cerevisiae SERF	1

Entities:

Entity 1, S. cerevisiae SERF 68 residues - Formula weight is not available

1

MET

ALA

ARG

GLY

ASN

GLN

ARG

ASP

LEU

ALA

2

ARG

GLN

LYS

ASN

LEU

LYS

GLN

LYS

ASP

3

MET

ALA

LYS

ASN

GLN

LYS

SER

GLY

ASP

4

PRO

LYS

ARG

MET

GLU

SER

ASP

ALA

GLU

5

ILE

LEU

ARG

GLN

LYS

GLN

ALA

ASP

6

ALA

ARG

GLU

ALA

GLU

LYS

LEU

GLU

LYS

7

LEU

LYS

ALA

GLU

LYS

THR

ARG

Samples:

sample_1: S. cerevisiae SERF, [U-100% 15N], 200 uM; sodium chloride 50 mM; potassium phosphate 20 mM

sample_conditions_1: ionic strength: 0.07 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks