BMRB Entry 5103
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5103
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NMR-STAR v3 text file.
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Citation: Lee, Y.; Volk, David; Thiviyanathan, Varatharasa; Kleerekoper, Q.; Gribenko, A.; Zhang, S.; Gorenstein, David; Makhatadze, G.; Luxon, Bruce. "NMR structure of the Apo-S100P protein" J. Biomol. NMR 29, 399-402 (2004).
PubMed: 15213440
Assembly members:
human s100p, polymer, 95 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
human s100p: MTELEAAMGMIIDVFSRYSG
SEGSTQTLTKGELKVLMEKE
LPGFLQSGKDKDAVDKLLKD
LDANGDAQVDFSEFIVFVAA
ITSASHKYFEKTGLK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 324 |
| 15N chemical shifts | 91 |
| 1H chemical shifts | 542 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | s100p subunit 1 | 1 |
| 2 | s100p subunit 2 | 1 |
Entities:
Entity 1, s100p subunit 1 95 residues - Formula weight is not available
| 1 | MET | THR | GLU | LEU | GLU | ALA | ALA | MET | GLY | MET | ||||
| 2 | ILE | ILE | ASP | VAL | PHE | SER | ARG | TYR | SER | GLY | ||||
| 3 | SER | GLU | GLY | SER | THR | GLN | THR | LEU | THR | LYS | ||||
| 4 | GLY | GLU | LEU | LYS | VAL | LEU | MET | GLU | LYS | GLU | ||||
| 5 | LEU | PRO | GLY | PHE | LEU | GLN | SER | GLY | LYS | ASP | ||||
| 6 | LYS | ASP | ALA | VAL | ASP | LYS | LEU | LEU | LYS | ASP | ||||
| 7 | LEU | ASP | ALA | ASN | GLY | ASP | ALA | GLN | VAL | ASP | ||||
| 8 | PHE | SER | GLU | PHE | ILE | VAL | PHE | VAL | ALA | ALA | ||||
| 9 | ILE | THR | SER | ALA | SER | HIS | LYS | TYR | PHE | GLU | ||||
| 10 | LYS | THR | GLY | LEU | LYS |
Samples:
sample_1: human s100p, [U-13C; U-15N], 2.3 – 3.0 mM
Condition_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | not available | not available | not available |
| 1H-13C NOESY | not available | not available | not available |
| HCCH TOCSY | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNHA | not available | not available | not available |
| HCACOCANH | not available | not available | not available |
| HNCO | not available | not available | not available |
| C(CO)NH | not available | not available | not available |
Software:
FELIX v97 - peak assignments, peak picking
NMR spectrometers:
- Varian UnityPlus 600 MHz
- Varian UnityPlus 750 MHz
Related Database Links:
| PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks