BMRB Entry 51023

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51023

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Title:
FLN5 A3A3
Deposition date:
2021-07-22
Original release date:
2021-08-23
Authors:
Cassaignau, Anais; Wlodarski, Tomasz; Chan, Sammy; Woodburn, Lauren; Vujkovic Bukvin, Ivana; Streit, Julian; Cabrita, Lisa; Waudby, Christopher; Christodoulou, John
Citation:

Citation: Cassaignau, Anais; Wlodarski, Tomasz; Chan, Sammy; Woodburn, Lauren; Vujkovic Bukvin, Ivana; Streit, Julian; Cabrita, Lisa; Waudby, Christopher; Christodoulou, John. "Interactions between nascent proteins and the ribosome surface inhibit co-translational folding"  Nat. Chem. 13, 1214-1220 (2021).
PubMed: 34650236

Assembly members:

Assembly members:
entity_1, polymer, 150 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Dictyostelium discoideum   Taxonomy ID: 44689   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Dictyostelium discoideum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pLDC

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MHHHHHHASKPAPSAEHSYA EGEGNVKVADNAPAEATIAA VDTKGVARTDGGDPFEVAIN GPDGLVVDAKVTDNNDGTAG VVADAPVEGNANVNVTLRGN PIKNMPIDVKCIEGPIDVKV IEGPIDVKVIEGPIDVKVIE GPIDVKCIEG

Data sets:
Data typeCount
15N chemical shifts96
1H chemical shifts96

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FLN5 A3A31

Entities:

Entity 1, FLN5 A3A3 150 residues - Formula weight is not available

1   METHISHISHISHISHISHISALASERLYS
2   PROALAPROSERALAGLUHISSERTYRALA
3   GLUGLYGLUGLYASNVALLYSVALALAASP
4   ASNALAPROALAGLUALATHRILEALAALA
5   VALASPTHRLYSGLYVALALAARGTHRASP
6   GLYGLYASPPROPHEGLUVALALAILEASN
7   GLYPROASPGLYLEUVALVALASPALALYS
8   VALTHRASPASNASNASPGLYTHRALAGLY
9   VALVALALAASPALAPROVALGLUGLYASN
10   ALAASNVALASNVALTHRLEUARGGLYASN
11   PROILELYSASNMETPROILEASPVALLYS
12   CYSILEGLUGLYPROILEASPVALLYSVAL
13   ILEGLUGLYPROILEASPVALLYSVALILE
14   GLUGLYPROILEASPVALLYSVALILEGLU
15   GLYPROILEASPVALLYSCYSILEGLUGLY

Samples:

sample_1: FLN5 A3A3, [U-100% 15N], 150 uM; HEPES 10 mM; NH4Cl 30 mM; MgCl2 12 mM; EDTA 5 mM; DSS 0.001%; D2O 10%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HMQCsample_1isotropicsample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks