BMRB Entry 5102

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5102

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

A helical region in the C-terminus of small-conductance Ca2+-activated K+ channels controls assembly with apo-calmodulin

Deposition date:

2001-08-08

Original release date:

2002-02-04

Authors:

Wissmann, Ralph; Bildl, Wolfgang; Neumann, Heinz; Rivard, Andre; Kloecker, Nikolaj; Weitz, Dietmar; Schulte, Uwe; Adelman, John; Bentrop, Detlef; Fakler, Bernd

Citation:

Citation: Wissmann, Ralph; Bildl, Wolfgang; Neumann, Heinz; Rivard, Andre; Kloecker, Nikolaj; Weitz, Dietmar; Schulte, Uwe; Adelman, John; Bentrop, Detlef; Fakler, Bernd. "A helical region in the C-terminus of small-conductance Ca2+-activated K+ channels controls assembly with apo-calmodulin" J. Biol. Chem. 277, 4558-4564 (2002).

Assembly members:

Assembly members:
SK2 calmodulin binding domain, polymer, 102 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET23B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SK2 calmodulin binding domain: MGRKLELTKAEKHVHNFMMD TQLTKRVKNAAANVLRETWL IYKNTKLVKKIDHAKVRKHQ RKFLQAIHQLRSVKMEQRKL NDQANTLVDLAKTQLEHHHH HH

Data sets:

assigned_chemical_shifts
coupling_constants
- J_values_set_1

Data type	Count
13C chemical shifts	342
15N chemical shifts	343
1H chemical shifts	871
coupling constants	37

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CaMBD	1

Entities:

Entity 1, CaMBD 102 residues - Formula weight is not available

1

MET

GLY

ARG

LYS

LEU

GLU

LEU

THR

LYS

ALA

2

GLU

LYS

HIS

VAL

HIS

ASN

PHE

MET

ASP

3

THR

GLN

LEU

THR

LYS

ARG

VAL

LYS

ASN

ALA

4

ALA

ASN

VAL

LEU

ARG

GLU

THR

TRP

LEU

5

ILE

TYR

LYS

ASN

THR

LYS

LEU

VAL

LYS

6

ILE

ASP

HIS

ALA

LYS

VAL

ARG

LYS

HIS

GLN

7

ARG

LYS

PHE

LEU

GLN

ALA

ILE

HIS

GLN

LEU

8

ARG

SER

VAL

LYS

MET

GLU

GLN

ARG

LYS

LEU

9

ASN

ASP

GLN

ALA

ASN

THR

LEU

VAL

ASP

LEU

10

ALA

LYS

THR

GLN

LEU

GLU

HIS

11

HIS

Samples:

sample_1: SK2 calmodulin binding domain, [U-98% 15N], 0.5 – 1.2 mM; NaCl 250 mM

sample_2: SK2 calmodulin binding domain, [U-98% 13C; U-98% 15N], 0.5 – 1.0 mM; NaCl 250 mM

Ex-cond_1: ionic strength: 0.25 M; pH: 3.5 na; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY	not available	not available	not available
1H-15N TOCSY	not available	not available	not available
HNCA	not available	not available	not available
HNCO	not available	not available	not available
HN(CA)CO	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
HNHA	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Bruker Avance 600 MHz

PDB	1G4Y 1KKD 1QX7 4G27 4G28 4J9Y 4J9Z 4QNH
DBJ	BAC28176 BAC34231 BAD08234 BAE28732 BAF82637
EMBL	CAH90265
GB	AAB09563 AAF21783 AAG16728 AAH15371 AAI17455
REF	NP_001125117 NP_001265133 NP_062187 NP_067627 NP_536713
SP	P58390 P70604 Q9H2S1
TPG	DAA25921
AlphaFold	P58390 P70604 Q9H2S1