BMRB Entry 51018

Name: Backbone 1H, 13C and 15N chemical shift assignments for the C-Terminal phosphorylated intrinsically disordered Gab1
Published: 2021-08-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51018

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N chemical shift assignments for the C-Terminal phosphorylated intrinsically disordered Gab1

Deposition date:

2021-07-15

Original release date:

2021-08-16

Authors:

Gruber, Tobias

Citation:

Citation: Gruber, Tobias; Lewitzky, Marc; Machner, Lisa; Weininger, Ulrich; Feller, Stephan M.; Balbach, Jochen. "Macromolecular crowding induces a binding competent transient structure in intrinsically disordered Gab1" J. Mol. Biol. 434, 167407-167407 (2021).
PubMed: 34929201

Assembly members:

Assembly members:
entity_1, polymer, 82 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET42a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SSPMIKPKGDKQVEYLDLDL DSGKSTPPRKQKSSGSGSSV ADERVDYVVVDQQKTLALKS TREAWTDGRQSTESETPAKS VK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	202
15N chemical shifts	69
1H chemical shifts	69

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C-Terminus of Gab1	1

Entities:

Entity 1, C-Terminus of Gab1 82 residues - Formula weight is not available

1

SER

PRO

MET

ILE

LYS

PRO

LYS

GLY

ASP

2

LYS

GLN

VAL

GLU

TYR

LEU

ASP

LEU

ASP

LEU

3

ASP

SER

GLY

LYS

SER

THR

PRO

ARG

LYS

4

GLN

LYS

SER

GLY

SER

GLY

SER

VAL

5

ALA

ASP

GLU

ARG

VAL

ASP

TYR

VAL

6

ASP

GLN

LYS

THR

LEU

ALA

LEU

LYS

SER

7

THR

ARG

GLU

ALA

TRP

THR

ASP

GLY

ARG

GLN

8

SER

THR

GLU

SER

GLU

THR

PRO

ALA

LYS

SER

9

VAL

LYS

Samples:

sample_1: C-Terminus of Gab1 Tyr-phosphorylated, [U-100% 13C; U-100% 15N], 0.9 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

NMRbox - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks