BMRB Entry 51018

Title:
Backbone 1H, 13C and 15N chemical shift assignments for the C-Terminal phosphorylated intrinsically disordered Gab1
Deposition date:
2021-07-15
Original release date:
2021-08-16
Authors:
Gruber, Tobias
Citation:

Citation: Gruber, Tobias; Lewitzky, Marc; Machner, Lisa; Weininger, Ulrich; Feller, Stephan M.; Balbach, Jochen. "Macromolecular crowding induces a binding competent transient structure in intrinsically disordered Gab1"  J. Mol. Biol. 434, 167407-167407 (2021).
PubMed: 34929201

Assembly members:

Assembly members:
entity_1, polymer, 82 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET42a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts202
15N chemical shifts69
1H chemical shifts69

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C-Terminus of Gab11

Entities:

Entity 1, C-Terminus of Gab1 82 residues - Formula weight is not available

1   SERSERPROMETILELYSPROLYSGLYASP
2   LYSGLNVALGLUTYRLEUASPLEUASPLEU
3   ASPSERGLYLYSSERTHRPROPROARGLYS
4   GLNLYSSERSERGLYSERGLYSERSERVAL
5   ALAASPGLUARGVALASPTYRVALVALVAL
6   ASPGLNGLNLYSTHRLEUALALEULYSSER
7   THRARGGLUALATRPTHRASPGLYARGGLN
8   SERTHRGLUSERGLUTHRPROALALYSSER
9   VALLYS

Samples:

sample_1: C-Terminus of Gab1 Tyr-phosphorylated, [U-100% 13C; U-100% 15N], 0.9 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

NMRbox - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks