BMRB Entry 51016

Name: Olduvai domain CON1 assignments at 5C
Published: 2022-02-18

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51016

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Olduvai domain CON1 assignments at 5C

Deposition date:

2021-07-13

Original release date:

2022-02-18

Authors:

Paukovich, Natasia; Issaian, Aaron; Henen, Morkos; Vogeli, Beat

Citation:

Citation: Paukovich, Natasia; Henen, Morkos; Hussain, Alya; Issaian, Aaron; Sikela, James; Hansen, Kirk; Vogeli, Beat. "Solution NMR backbone assignments of disordered Olduvai protein domain CON1 employing Ha-detected experiments" Biomol. NMR Assign. 16, 113-119 (2022).
PubMed: 35098449

Assembly members:

Assembly members:
entity_1, polymer, 91 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHLmMBP-11 Sec-mMBP-TEV-6xHis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SAAAASSASVQVEVAEKVQK SSAPREMQKAEEKEVPEDSL EECAITCSNSHGPYDSNQPH KKTKITFEEDKVDSTLIGSS SHVLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	152
15N chemical shifts	71
1H chemical shifts	71

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CON1	1

Entities:

Entity 1, CON1 91 residues - Formula weight is not available

1

SER

ALA

SER

ALA

SER

VAL

2

GLN

VAL

GLU

VAL

ALA

GLU

LYS

VAL

GLN

LYS

3

SER

ALA

PRO

ARG

GLU

MET

GLN

LYS

ALA

4

GLU

LYS

GLU

VAL

PRO

GLU

ASP

SER

LEU

5

GLU

CYS

ALA

ILE

THR

CYS

SER

ASN

SER

6

HIS

GLY

PRO

TYR

ASP

SER

ASN

GLN

PRO

HIS

7

LYS

THR

LYS

ILE

THR

PHE

GLU

ASP

8

LYS

VAL

ASP

SER

THR

LEU

ILE

GLY

SER

9

SER

HIS

VAL

LEU

GLU

HIS

10

HIS

Samples:

sample_1: CON1, [U-100% 13C; U-100% 15N], 500 uM; sodium phosphate 50 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4.2 - chemical shift assignment, data analysis

NMRPipe - processing

NMRFAM-SPARKY - data analysis

NMR spectrometers:

Varian INOVA 900 MHz
Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks