BMRB Entry 51015

Title:
Olduvai domain CON1 assignments at 25C
Deposition date:
2021-07-13
Original release date:
2022-02-18
Authors:
Paukovich, Natasia; Issaian, Aaron; Henen, Morkos; Vogeli, Beat
Citation:

Citation: Paukovich, Natasia; Henen, Morkos; Hussain, Alya; Issaian, Aaron; Sikela, James; Hansen, Kirk; Vogeli, Beat. "Solution NMR backbone assignments of disordered Olduvai protein domain CON1 employing Ha-detected experiments"  Biomol. NMR Assign. 16, 113-119 (2022).
PubMed: 35098449

Assembly members:

Assembly members:
entity_1, polymer, 91 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHLmMBP-11 Sec-mMBP-TEV-6xHis

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts222
15N chemical shifts79
1H chemical shifts151

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CON11

Entities:

Entity 1, CON1 91 residues - Formula weight is not available

1   SERALAALAALAALASERSERALASERVAL
2   GLNVALGLUVALALAGLULYSVALGLNLYS
3   SERSERALAPROARGGLUMETGLNLYSALA
4   GLUGLULYSGLUVALPROGLUASPSERLEU
5   GLUGLUCYSALAILETHRCYSSERASNSER
6   HISGLYPROTYRASPSERASNGLNPROHIS
7   LYSLYSTHRLYSILETHRPHEGLUGLUASP
8   LYSVALASPSERTHRLEUILEGLYSERSER
9   SERHISVALLEUGLUHISHISHISHISHIS
10   HIS

Samples:

sample_1: CON1, [U-100% 13C; U-100% 15N], 500 uM; sodium phosphate 50 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HA(CA)CONsample_1isotropicsample_conditions_1
3D iHACANCOsample_1isotropicsample_conditions_1
3D HACA(CON)CAHAsample_1isotropicsample_conditions_1
3D HACACONsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.4.2 - chemical shift assignment, data analysis

NMRPipe - processing

NMRFAM-SPARKY - data analysis

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks