BMRB Entry 51014
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51014
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Citation: de Araujo, Talita; Lima, Luis Mauricio; Almeida, Marcius. "Unambiguous characterization of PEGylation site on human amylin by 2D NMR" J. Biomol. NMR ., .-..
Assembly members:
entity_1, polymer, 38 residues, Formula weight is not available
entity_PEG, non-polymer, 106.120 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: KCNTATCATQRLANFLVHSS
NNFGAILSSTNVGSNTYX
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 88 |
| 1H chemical shifts | 231 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Human Amylin | 1 |
| 2 | polyethylene glycol | 2 |
Entities:
Entity 1, Human Amylin 38 residues - Formula weight is not available
| 1 | LYS | CYS | ASN | THR | ALA | THR | CYS | ALA | THR | GLN | ||||
| 2 | ARG | LEU | ALA | ASN | PHE | LEU | VAL | HIS | SER | SER | ||||
| 3 | ASN | ASN | PHE | GLY | ALA | ILE | LEU | SER | SER | THR | ||||
| 4 | ASN | VAL | GLY | SER | ASN | THR | TYR | NH2 |
Entity 2, polyethylene glycol - C4 H10 O3 - 106.120 Da.
| 1 | PEG |
Samples:
sample_1: Human Amylin HNz-PEGylated 0.5 mM; sodium acetate 10 mM
sample_conditions_1: pH: 4.5; temperature: 285 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN vv4.0.6 - collection, data analysis, processing
CARA vv1.9.1.5 - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker AVANCE III 400 MHz