BMRB Entry 5100
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5100
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Matthews, Jacqueline; Visvader, Jane; Mackay, Joel. "1H, 15N and 13C Assignments of FLIN2, an Intramolecular LMO2:ldb1 Complex" J. Biomol. NMR 21, 385-386 (2001).
PubMed: 11824760
Assembly members:
Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2, polymer, 114 residues, 12589 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Vector: pGEX-2T
Entity Sequences (FASTA):
Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2: GSLLTCGGCQQNIGDRYFLK
AIDQYWHEDCLSCDLCGCRL
GEVGRRLYYKLGRKLCRRDY
LRLGGSGGHMGSGGDVMVVG
EPTLMGGEFGDEDERLITRL
ENTQFDAANGIDDE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 344 |
| 15N chemical shifts | 115 |
| 1H chemical shifts | 1316 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FLIN2 | 1 |
| 2 | ZINC (II) ION, I | 2 |
| 3 | ZINC (II) ION, II | 2 |
Entities:
Entity 1, FLIN2 114 residues - 12589 Da.
| 1 | GLY | SER | LEU | LEU | THR | CYS | GLY | GLY | CYS | GLN | ||||
| 2 | GLN | ASN | ILE | GLY | ASP | ARG | TYR | PHE | LEU | LYS | ||||
| 3 | ALA | ILE | ASP | GLN | TYR | TRP | HIS | GLU | ASP | CYS | ||||
| 4 | LEU | SER | CYS | ASP | LEU | CYS | GLY | CYS | ARG | LEU | ||||
| 5 | GLY | GLU | VAL | GLY | ARG | ARG | LEU | TYR | TYR | LYS | ||||
| 6 | LEU | GLY | ARG | LYS | LEU | CYS | ARG | ARG | ASP | TYR | ||||
| 7 | LEU | ARG | LEU | GLY | GLY | SER | GLY | GLY | HIS | MET | ||||
| 8 | GLY | SER | GLY | GLY | ASP | VAL | MET | VAL | VAL | GLY | ||||
| 9 | GLU | PRO | THR | LEU | MET | GLY | GLY | GLU | PHE | GLY | ||||
| 10 | ASP | GLU | ASP | GLU | ARG | LEU | ILE | THR | ARG | LEU | ||||
| 11 | GLU | ASN | THR | GLN | PHE | ASP | ALA | ALA | ASN | GLY | ||||
| 12 | ILE | ASP | ASP | GLU |
Entity 2, ZINC (II) ION, I - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO20.3 – 0.5 mM; ZINC (II) ION0.6 – 1.0 mM
sample_2: Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2, [U-95% 15N], 0.3 – 0.5 mM; ZINC (II) ION0.6 – 1.0 mM
sample_3: Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2, [U-95% 13C; U-90% 15N], 0.3 – 0.5 mM; ZINC (II) ION0.6 – 1.0 mM
cond_1: ionic strength: 0.07 M; pH: 7.0; temperature: 298 K
cond_2: ionic strength: 0.07 M; pH: 5.6; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H TOCSY | not available | not available | not available |
| 1H-1H COSY | not available | not available | not available |
| 1H-1H NOESY | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| CC(CO)NH TOCSY | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| HNHA | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| 1H-15N NOESY | not available | not available | not available |
| 1H-15N HSQC | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- BRUKER DMX 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks