BMRB Entry 50997

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50997
MolProbity Validation Chart

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Title:
Zinc finger
Deposition date:
2021-06-30
Original release date:
2022-06-27
Authors:
Overbeck, Jan; Sprangers, Remco
Citation:

Citation: Overbeck, Jan; Stelzig, David; Fuchs, Anna-Lisa; Wurm, Jan Philip; Sprangers, Remco. "Observation of conformational changes that underlie the catalytic cycle of Xrn2"  Nat. Chem. Biol. 18, 1152-1160 (2022).
PubMed: 36008487

Assembly members:

Assembly members:
entity_1, polymer, 31 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Chaetomium thermophilum   Taxonomy ID: 209285   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Chaetomium thermophilum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETGB1a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGEAKARLCKLCGQKGHDER SCKGEAKQKQG

Data sets:
Data typeCount
13C chemical shifts117
15N chemical shifts30
1H chemical shifts176

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1zinc finger polypeptide1
2Zinc ion2

Entities:

Entity 1, zinc finger polypeptide 31 residues - Formula weight is not available

1   GLYGLYGLUALALYSALAARGLEUCYSLYS
2   LEUCYSGLYGLNLYSGLYHISASPGLUARG
3   SERCYSLYSGLYGLUALALYSGLNLYSGLN
4   GLY

Entity 2, Zinc ion - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Zinc finger, [U-13C; U-15N], 0.75 mM; Zn2+ 0.75 mM; HEPES 25 mM; NaCl 125 mM; NaN3 0.03%

sample_conditions_1: ionic strength: 0.125 M; pH: 7.3; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D 15N-separated NOESYsample_1isotropicsample_conditions_1
3D 13C-separated NOESYsample_1isotropicsample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks