BMRB Entry 50994

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50994
MolProbity Validation Chart

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Title:
Backbone 1H, 15N and 13C resonance assignments of a non-structural protein NS2B of Zika virus
Deposition date:
2021-06-23
Original release date:
2021-12-13
Authors:
Penna, Beatriz; de Oliveira, Danielle Maria; Anobom, Cristiane; Valente, Ana Paula
Citation:

Citation: Penna, Beatriz; de Oliveira, Danielle; Anobom, Cristiane; Valente, Ana Paula. "Backbone 1 H, 15 N, and 13 C resonance assignments of the non-structural protein NS2B of Zika virus"  Biomolecular NMR Assignments 16, 31-35 (2022).
PubMed: 34817802

Assembly members:

Assembly members:
entity_1, polymer, 160 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Zika Virus   Taxonomy ID: 64320   Superkingdom: Viruses   Kingdom: not available   Genus/species: Flavivirus Zika Virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH MGSENLYFQGSWPPSEVLTA VGLICALAGGFAKADIEMAG PMAAVGLLIVSYVVSGKSVD MYIERAGDITWEKDAEVTGN SPRLDVALDESGDFSLVEED GPPMREIILKVVLMAICGMN PIAIPFAAGAWYVYVKTGKR

Data sets:
Data typeCount
13C chemical shifts388
15N chemical shifts126
1H chemical shifts126

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NS2B Protein1

Entities:

Entity 1, NS2B Protein 160 residues - Formula weight is not available

Residues 1-30 represent a hexa-histidine tag, thrombin and TEV cleavage sites.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METGLYSERGLUASNLEUTYRPHEGLNGLY
4   SERTRPPROPROSERGLUVALLEUTHRALA
5   VALGLYLEUILECYSALALEUALAGLYGLY
6   PHEALALYSALAASPILEGLUMETALAGLY
7   PROMETALAALAVALGLYLEULEUILEVAL
8   SERTYRVALVALSERGLYLYSSERVALASP
9   METTYRILEGLUARGALAGLYASPILETHR
10   TRPGLULYSASPALAGLUVALTHRGLYASN
11   SERPROARGLEUASPVALALALEUASPGLU
12   SERGLYASPPHESERLEUVALGLUGLUASP
13   GLYPROPROMETARGGLUILEILELEULYS
14   VALVALLEUMETALAILECYSGLYMETASN
15   PROILEALAILEPROPHEALAALAGLYALA
16   TRPTYRVALTYRVALLYSTHRGLYLYSARG

Samples:

sample_1: NS2B, [U-13C; U-15N; U-2H], 0.5 mM; sodium phosphate 20 mM; sodium azide 5 mM; beta-mercaptoethanol 2 mM; SDS 0.1%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN vTopSpin3.1 - processing

NMRPipe - processing

CcpNMR vAnalysis v.2.4.2 - data analysis

NMRbox - data analysis

NMR spectrometers:

  • Bruker AVANCE III 900 MHz

Related Database Links:

NCBI AEN75265.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks