BMRB Entry 50983

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50983

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Title:
AMTC24-5
Deposition date:
2021-06-17
Original release date:
2021-10-07
Authors:
Preusske, Nils; Sonnichsen, Frank
Citation:

Citation: Preusske, Nils; Lipfert, Matthias; Rothemund, Sven; Leippe, Matthias; Sonnichsen, Frank. "Designed Trp-Cage Proteins with Antimicrobial Activity and Enhanced Stability"  Biochemistry 60, 3187-3199 (2021).
PubMed: 34613690

Assembly members:

Assembly members:
entity_1, polymer, 24 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GIFKRLYKQWLKDGGPSSGR PPPK

Data sets:
Data typeCount
13C chemical shifts85
1H chemical shifts147

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AMTC24-51

Entities:

Entity 1, AMTC24-5 24 residues - Formula weight is not available

1   GLYILEPHELYSARGLEUTYRLYSGLNTRP
2   LEULYSASPGLYGLYPROSERSERGLYARG
3   PROPROPROLYS

Samples:

sample_1: AMTC24-5 370 ± 37.0 uM; DSS 7.6 ± 0.76 uM

sample_conditions_1: pH: 5.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

NMRViewJ v9.2.0-b24 - chemical shift assignment

TOPSPIN v4.0.6 - collection

NMR spectrometers:

  • Bruker AVANCE II 600.1 MHz MHz