BMRB Entry 50981
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50981
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Citation: Preusske, Nils; Lipfert, Matthias; Rothemund, Sven; Leippe, Matthias; Sonnichsen, Frank. "Designed Trp-Cage Proteins with Antimicrobial Activity and Enhanced Stability" Biochemistry 60, 3187-3199 (2021).
PubMed: 34613690
Assembly members:
entity_1, polymer, 26 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: RIKDFLRKYAQWLADGGPSS
GRPPPK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 87 |
| 1H chemical shifts | 160 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AMTC26-4 | 1 |
Entities:
Entity 1, AMTC26-4 26 residues - Formula weight is not available
| 1 | ARG | ILE | LYS | ASP | PHE | LEU | ARG | LYS | TYR | ALA | ||||
| 2 | GLN | TRP | LEU | ALA | ASP | GLY | GLY | PRO | SER | SER | ||||
| 3 | GLY | ARG | PRO | PRO | PRO | LYS |
Samples:
sample_1: AMTC26-4 376 ± 37.6 uM; DSS 7.6 ± 0.76 uM
sample_conditions_1: pH: 5.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRViewJ v9.2.0-b24 - chemical shift assignment
TOPSPIN v4.0.6 - collection
NMR spectrometers:
- Bruker AVANCE II 600.1 MHz MHz