BMRB Entry 50980
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50980
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NMR-STAR v3 text file.
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Citation: Preusske, Nils; Lipfert, Matthias; Rothemund, Sven; Leippe, Matthias; Sonnichsen, Frank. "Designed Trp-Cage Proteins with Antimicrobial Activity and Enhanced Stability" Biochemistry 60, 3187-3199 (2021).
PubMed: 34613690
Assembly members:
entity_1, polymer, 31 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: KRIVQRIKDFLRKYAQWLAD
GGPSSGRPPPK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 109 |
| 1H chemical shifts | 201 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AMTC31-6 | 1 |
Entities:
Entity 1, AMTC31-6 31 residues - Formula weight is not available
| 1 | LYS | ARG | ILE | VAL | GLN | ARG | ILE | LYS | ASP | PHE | ||||
| 2 | LEU | ARG | LYS | TYR | ALA | GLN | TRP | LEU | ALA | ASP | ||||
| 3 | GLY | GLY | PRO | SER | SER | GLY | ARG | PRO | PRO | PRO | ||||
| 4 | LYS |
Samples:
sample_1: AMTC31-6 400 ± 40 uM; DSS 7.6 ± 0.76 uM
sample_conditions_1: pH: 5.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRViewJ v9.2.0-b24 - chemical shift assignment
TOPSPIN v4.0.6 - collection
NMR spectrometers:
- Bruker AVANCE II 600.1 MHz MHz