BMRB Entry 50977

Title:
Human obscurin Ig12
Deposition date:
2021-06-16
Original release date:
2022-10-24
Authors:
Mauriello, Gianna; Wright, Nathan
Citation:

Citation: Mauriello, Gianna; Moncure, Grace; Nowzari, Roujon; Miller, Callie; Wright, Nathan. "The N-terminus of obscurin is flexible in solution"  Proteins 91, 485-496 (2023).
PubMed: 36306263

Assembly members:

Assembly members:
entity_1, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24a

Data sets:
Data typeCount
13C chemical shifts272
15N chemical shifts85
1H chemical shifts561

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Human obscurin Ig121

Entities:

Entity 1, Human obscurin Ig12 101 residues - Formula weight is not available

1   METLYSMETMETPHEALALYSGLUGLNSER
2   VALHISASNGLUVALGLNALAGLUALAGLY
3   ALASERALAMETLEUSERCYSGLUVALALA
4   GLNALAGLNTHRGLUVALTHRTRPTYRLYS
5   ASPGLYLYSLYSLEUSERSERSERSERLYS
6   VALGLYMETGLUVALLYSGLYCYSTHRARG
7   ARGLEUVALLEUPROGLNALAGLYLYSALA
8   ASPALAGLYGLUTYRSERCYSGLUALAGLY
9   GLYGLNARGVALSERPHEHISLEUHISILE
10   THRGLUPROLEUGLUHISHISHISHISHIS
11   HIS

Samples:

sample_1: Human obscurin Ig12, [U-100% 15N], 1 mM; TRIS 50 mM; NaCl 20 mM; NaN3 0.35 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
1H-15N heteronoesample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

SPARKY - chemical shift assignment

X-PLOR NIH - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks