BMRB Entry 50976

Name: Human obscurin Ig13
Published: 2022-10-24

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PDB ID: 7r67
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50976
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Human obscurin Ig13

Deposition date:

2021-06-16

Original release date:

2022-10-24

Authors:

Moncure, Grace; Wright, Nathan

Citation:

Citation: Mauriello, Gianna; Moncure, Grace; Nowzari, Roujon; Miller, Callie; Wright, Nathan. "The N-terminus of obscurin is flexible in solution" Proteins 91, 485-496 (2023).
PubMed: 36306263

Assembly members:

Assembly members:
entity_1, polymer, 103 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MEPKGVFAKEQSVHNEVQAE AGTTAMLSCEVAQPQTEVTW YKDGKKLSSSSKVRMEVKGC TRRLVVQQVGKADAGEYSCE AGGQRVSFQLHITEPLEHHH HHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	342
15N chemical shifts	88
1H chemical shifts	578

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Human obscurin Ig13	1

Entities:

Entity 1, Human obscurin Ig13 103 residues - Formula weight is not available

1

MET

GLU

PRO

LYS

GLY

VAL

PHE

ALA

LYS

GLU

2

GLN

SER

VAL

HIS

ASN

GLU

VAL

GLN

ALA

GLU

3

ALA

GLY

THR

ALA

MET

LEU

SER

CYS

GLU

4

VAL

ALA

GLN

PRO

GLN

THR

GLU

VAL

THR

TRP

5

TYR

LYS

ASP

GLY

LYS

LEU

SER

6

SER

LYS

VAL

ARG

MET

GLU

VAL

LYS

GLY

CYS

7

THR

ARG

LEU

VAL

GLN

VAL

GLY

8

LYS

ALA

ASP

ALA

GLY

GLU

TYR

SER

CYS

GLU

9

ALA

GLY

GLN

ARG

VAL

SER

PHE

GLN

LEU

10

HIS

ILE

THR

GLU

PRO

LEU

GLU

HIS

11

HIS

Samples:

sample_1: Human obscurin Ig13, [U-100% 15N], 1 mM; TRIS 50 mM; NaCl 20 mM; NaN3 0.35 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
1H-15N heteronoe	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

SPARKY - chemical shift assignment

X-PLOR NIH - refinement

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks