BMRB Entry 50966

Name: 1H, 15N, 13C resonnance assigments and secondary structure of PulL, a component of the Klebsiella oxytoca type II secretion system
Published: 2021-08-27

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50966

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, 13C resonnance assigments and secondary structure of PulL, a component of the Klebsiella oxytoca type II secretion system

Deposition date:

2021-06-09

Original release date:

2021-08-27

Authors:

Dazzoni, Regine; Lopez-Castilla, Aracelys; Cordier, Florence; Bardiaux, Benjamin; Nilges, Michael; Izadi-Pruneyre, Nadia

Citation:

Citation: Dazzoni, Regine; Lopez-Castilla, Aracelys; Cordier, Florence; Bardiaux, Benjamin; Nilges, Michael; Francetic, Olivera; Izadi-Pruneyre, Nadia. "1H, 15N and 13C resonance assignments of the C-terminal domain of PulL, a component of the Klebsiella oxytoca type II secretion system" Biomol. NMR Assignments 15, 455-459 (2021).
PubMed: 34410621

Assembly members:

Assembly members:
entity_1, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Klebsiella oxytoca Taxonomy ID: 571 Superkingdom: Bacteria Kingdom: not available Genus/species: Klebsiella oxytoca

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMalp2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SNAGARPALISRLGALQQII DDTPGIRLRTLSFDAARNAL QLEISAVSSQALEQFSQRAR ARFRVQTGEMKPRADGIEGR LTLEGNDA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	317
15N chemical shifts	89
1H chemical shifts	471

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PulL CTD	1

Entities:

Entity 1, PulL CTD 88 residues - Formula weight is not available

1

SER

ASN

ALA

GLY

ALA

ARG

PRO

ALA

LEU

ILE

2

SER

ARG

LEU

GLY

ALA

LEU

GLN

ILE

3

ASP

THR

PRO

GLY

ILE

ARG

LEU

ARG

THR

4

LEU

SER

PHE

ASP

ALA

ARG

ASN

ALA

LEU

5

GLN

LEU

GLU

ILE

SER

ALA

VAL

SER

GLN

6

ALA

LEU

GLU

GLN

PHE

SER

GLN

ARG

ALA

ARG

7

ALA

ARG

PHE

ARG

VAL

GLN

THR

GLY

GLU

MET

8

LYS

PRO

ARG

ALA

ASP

GLY

ILE

GLU

GLY

ARG

9

LEU

THR

LEU

GLU

GLY

ASN

ASP

ALA

Samples:

sample_1: PulL CTD, [U-100% 13C; U-100% 15N], 450 uM; Hepes 50 mM; NaCl 50 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HMQC	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

ANALYSIS - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks