BMRB Entry 50964

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50964

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
yRad6
Deposition date:
2021-06-07
Original release date:
2023-03-01
Authors:
Shukla, Prakash; Skalicky, Jack; Chandrasekharan, Mahesh
Citation:

Citation: Shukla, Prakash; Sinha, Dhiraj; Leng, Andrew; Bissell, Jesse; Thatipamula, Shravya; Ganguly, Rajarshi; Radmall, Kaitlin; Skalicky, Jack; Shrieve, Dennis; Chandrasekharan, Mahesh. "Mutations of Rad6 E2 ubiquitin-conjugating enzymes at alanine-126 in helix-3 affect ubiquitination activity and decrease enzyme stability"  J. Biol. Chem. 298, 102524-102524 (2022).
PubMed: 36162503

Assembly members:

Assembly members:
entity_1, polymer, 150 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Saccharomyces cerevisiae   Vector: unknown

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSTPARRRLMRDFKRMKEDA PPGVSASPLPDNVMVWNAMI IGPADTPYEDGTFRLLLEFD EEYPNKPPHVKFLSEMFHPN VYANGEICLDILQNRWTPTY DVASILTSIQSLFNDPNPAS PANVEAATLFKDHKSQYVKR VKETVEKSWE

Data sets:
Data typeCount
13C chemical shifts291
15N chemical shifts129
1H chemical shifts129

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1yRad61

Entities:

Entity 1, yRad6 150 residues - Formula weight is not available

1   METSERTHRPROALAARGARGARGLEUMET
2   ARGASPPHELYSARGMETLYSGLUASPALA
3   PROPROGLYVALSERALASERPROLEUPRO
4   ASPASNVALMETVALTRPASNALAMETILE
5   ILEGLYPROALAASPTHRPROTYRGLUASP
6   GLYTHRPHEARGLEULEULEUGLUPHEASP
7   GLUGLUTYRPROASNLYSPROPROHISVAL
8   LYSPHELEUSERGLUMETPHEHISPROASN
9   VALTYRALAASNGLYGLUILECYSLEUASP
10   ILELEUGLNASNARGTRPTHRPROTHRTYR
11   ASPVALALASERILELEUTHRSERILEGLN
12   SERLEUPHEASNASPPROASNPROALASER
13   PROALAASNVALGLUALAALATHRLEUPHE
14   LYSASPHISLYSSERGLNTYRVALLYSARG
15   VALLYSGLUTHRVALGLULYSSERTRPGLU

Samples:

sample_1: potassium phosphate 25 mM; TCEP 0.5 mM; Rad6, [U-100% 13C; U-100% 15N], 0.75 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks