BMRB Entry 50962

Name: Backbone 1H, 13C and 15N chemical shift assignment of C-terminal polybasic region of human Semaphorin 3A
Published: 2021-08-04

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50962

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N chemical shift assignment of C-terminal polybasic region of human Semaphorin 3A

Deposition date:

2021-06-07

Original release date:

2021-08-04

Authors:

Perez, Yolanda; Bonet, Roman; Corredor, Miriam; Domingo, Cecilia; Moure, Alejandra; Bujons, Jordi; Messeguer, Angel; Alfonso, Ignacio

Citation:

Citation: Perez, Yolanda; Bonet, Roman; Corredor, Miriam; Domingo, Cecilia; Moure, Alejandra; Messeguer, Angel; Bujons, Jordi; Alfonso, Ignacio. "Semaphorin 3A - Glycosaminoglycans interaction as therapeu-tic target for axonal regeneration" Pharmaceuticals (Basel) 14, 906-906 (2021).
PubMed: 34577606

Assembly members:

Assembly members:
entity_1, polymer, 48 residues, 5915 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET45

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AVWKRDRKQRRQRPGHTPGN SNKWKHLQENKKGRNRRTHE FERAPRSV

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	140
15N chemical shifts	47
1H chemical shifts	284

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C-terminal polybasic domain Sema3A	1

Entities:

Entity 1, C-terminal polybasic domain Sema3A 48 residues - 5915 Da.

1

ALA

VAL

TRP

LYS

ARG

ASP

ARG

LYS

GLN

ARG

2

ARG

GLN

ARG

PRO

GLY

HIS

THR

PRO

GLY

ASN

3

SER

ASN

LYS

TRP

LYS

HIS

LEU

GLN

GLU

ASN

4

LYS

GLY

ARG

ASN

ARG

THR

HIS

GLU

5

PHE

GLU

ARG

ALA

PRO

ARG

SER

VAL

Samples:

sample_1: C-terminal polybasic domain of Semaphorin 3A, [U-100% 13C; U-100% 15N], 1 mM; acetate 10 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 60 mM; pH: 5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.5.7 - collection, data analysis

CcpNMR v2.4 - chemical shift assignment, data analysis

NMR spectrometers:

Bruker Bruker 500 MHz Avance HDIII 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks