BMRB Entry 50955

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50955

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Title:
Backbone Chemical Shift Assignments of human glutathione peroxidase 4 (GPx4)
Deposition date:
2021-05-28
Original release date:
2021-09-08
Authors:
Labrecque, Courtney; Fuglestad, Brian
Citation:

Citation: Labrecque, Courtney; Fuglestad, Brian. "Electrostatic Drivers of GPx4 Interactions with Membrane, Lipids, and DNA"  Biochemistry 60, 2761-2772 (2021).
PubMed: 34492183

Assembly members:

Assembly members:
entity_1, polymer, 164 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pNIC28-Bsa4

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SMRCARSMHEFSAKDIDGHM VNLDKYRGFVCIVTNVASQG GKTEVNYTQLVDLHARYAEC GLRILAFPCNQFGKQEPGSN EEIKEFAAGYNVKFDMFSKI CVNGDDAHPLWKWMKIQPKG KGILGNAIKWNFTKFLIDKN GCVVKRYGPMEEPLVIEKDL PHYF

Data sets:
Data typeCount
13C chemical shifts474
15N chemical shifts154
1H chemical shifts154

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1GPx41

Entities:

Entity 1, GPx4 164 residues - Formula weight is not available

human, cytosolic GPx4, truncated at position 8, U46 mutated to G

1   SERMETARGCYSALAARGSERMETHISGLU
2   PHESERALALYSASPILEASPGLYHISMET
3   VALASNLEUASPLYSTYRARGGLYPHEVAL
4   CYSILEVALTHRASNVALALASERGLNGLY
5   GLYLYSTHRGLUVALASNTYRTHRGLNLEU
6   VALASPLEUHISALAARGTYRALAGLUCYS
7   GLYLEUARGILELEUALAPHEPROCYSASN
8   GLNPHEGLYLYSGLNGLUPROGLYSERASN
9   GLUGLUILELYSGLUPHEALAALAGLYTYR
10   ASNVALLYSPHEASPMETPHESERLYSILE
11   CYSVALASNGLYASPASPALAHISPROLEU
12   TRPLYSTRPMETLYSILEGLNPROLYSGLY
13   LYSGLYILELEUGLYASNALAILELYSTRP
14   ASNPHETHRLYSPHELEUILEASPLYSASN
15   GLYCYSVALVALLYSARGTYRGLYPROMET
16   GLUGLUPROLEUVALILEGLULYSASPLEU
17   PROHISTYRPHE

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks