BMRB Entry 50944

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50944

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Title:
Backbone and nearly complete side-chain chemical shift assignments of the human Uncharacterized protein CXorf51A
Deposition date:
2021-05-19
Original release date:
2021-08-28
Authors:
Wiedemann, Christoph; Obika, Kingsley Benjamin; Liebscher, Sandra; Jirschitzka, Jan; Ohlenschlager, Oliver; Bordusa, Frank
Citation:

Citation: Wiedemann, Christoph; Obika, Kingsley Benjamin; Liebscher, Sandra; Jirschitzka, Jan; Ohlenschlager, Oliver; Bordusa, Frank. "Backbone and nearly complete side-chain chemical shift assignments reveal the human uncharacterized protein CXorf51A as intrinsically disordered"  Biomol. NMR Assignments 15, 441-448 (2021).
PubMed: 34415548

Assembly members:

Assembly members:
entity_1, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH MAKVTSEPQKPNEDVDEQTP STSSTKGRKKGKTPRQRRSR SGVKGLKTTRKAKRPLRGSS SQKAGETNTPAGKPKKARGP ILRGRYHRLKEKMKKEEADK EQSETSVL

Data sets:
Data typeCount
13C chemical shifts509
15N chemical shifts108
1H chemical shifts794

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CX05A_HUMAN1

Entities:

Entity 1, CX05A_HUMAN 128 residues - Formula weight is not available

Residue numbering starts at with -19 because the first 20 residues originate from the pruification tag.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METALALYSVALTHRSERGLUPROGLNLYS
4   PROASNGLUASPVALASPGLUGLNTHRPRO
5   SERTHRSERSERTHRLYSGLYARGLYSLYS
6   GLYLYSTHRPROARGGLNARGARGSERARG
7   SERGLYVALLYSGLYLEULYSTHRTHRARG
8   LYSALALYSARGPROLEUARGGLYSERSER
9   SERGLNLYSALAGLYGLUTHRASNTHRPRO
10   ALAGLYLYSPROLYSLYSALAARGGLYPRO
11   ILELEUARGGLYARGTYRHISARGLEULYS
12   GLULYSMETLYSLYSGLUGLUALAASPLYS
13   GLUGLNSERGLUTHRSERVALLEU

Samples:

sample_1: CX05A_HUMAN, [U-99% 13C; U-99% 15N], 400 uM; D2O, [U-99% 2H], 10%; H2O 90%; sodium phosphate 10 mM; sodium chloride 150 mM; DSS 0.1 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 283.2 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.6.2 - collection, processing

CcpNMR v2.5.1 - chemical shift assignment, data analysis, peak picking

NMRbox - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Related Database Links:

UNP A0A1B0GTR3
AlphaFold A0A1B0GTR3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks