BMRB Entry 50940

Title:
Drosophila melanogaster atypical Tm1 (aTm1) residues 1-213
Deposition date:
2021-05-12
Original release date:
2021-06-14
Authors:
Hennig, Janosch
Citation:

Citation: Vaishali, Vaishali; Dimitrova-Paternoga, Lyudmila; Haubrich, Kevin; Sun, Mai; Ephrussi, Anne; Hennig, Janosch. "Validation and classification of RNA binding proteins identified by mRNA interactome capture"  RNA 27, 1173-1185 (2021).
PubMed: 34215685

Assembly members:

Assembly members:
entity_1, polymer, 214 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11d

Data sets:
Data typeCount
13C chemical shifts318
15N chemical shifts169
1H chemical shifts169

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1aTm1 (1-213)1

Entities:

Entity 1, aTm1 (1-213) 214 residues - Formula weight is not available

1   SERMETGLUHISASPASPSERSERTHRSER
2   GLYTHRGLYSERHISTHRALASERASNTHR
3   SERLEUVALPROALASERLEULYSARGARG
4   GLYHISGLNHISHISPROARGPHESERGLY
5   THRARGARGPROASNVALPROASNVALGLN
6   GLUILELEUALAALALEUTYRARGGLYASP
7   SERLYSSERALALEUSERASNLEUARGGLY
8   GLUTHRGLNPROGLUGLUGLUGLNPROGLN
9   GLNGLNTHRGLUGLUVALLEUPROPROSER
10   ARGSERTHRLEUSERLEUPROLEUSERGLU
11   SERVALTHRASNSERLEUGLYSERASNSER
12   PROTHRPROTHRASPGLUSERSERVALGLN
13   ASPGLUGLYALASERASNPROALAALAALA
14   LEUILEALAGLUASPALAALAPROPROALA
15   GLYTHRTHRTHRSERLYSSERLYSLYSLYS
16   LYSARGGLULYSGLYGLUARGSERGLULYS
17   SERASPLYSSERGLULYSSERASPARGLYS
18   LYSLYSSERSERGLYLYSLYSGLUARGSER
19   LYSARGSERASNPROMETGLUGLNSERSER
20   ASPSERLEUALATHRASPLEUSERALAGLY
21   ALAILEASPGLUGLYILEALALEUALAASP
22   ASPASPASPASN

Samples:

sample_1: aTm1 (1-213), [U-100% 15N], 200 uM; PO4 20 mM; NaCl 150 mM; TCEP 0.5 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CARA v1.9.1 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks