BMRB Entry 5094

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PDB ID: 1jjl
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5094
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemosensory Protein from Moth Mamestra brassicae. Expression and Secondary Structure from 1H and 15N NMR

Deposition date:

2001-08-02

Original release date:

2003-01-07

Authors:

Campanacci, V.; Mosbah, A.; Bornet, O.; Wechselberger, R.; Jacquin-Joly, E.; Cambillau, C.; Darbon, H.; Tegoni, M.

Citation:

Citation: Campanacci, V.; Mosbah, A.; Bornet, O.; Wechselberger, R.; Jacquin-Joly, E.; Cambillau, C.; Darbon, H.; Tegoni, M.. "Chemosensory Protein from Moth Mamestra brassicae. Expression and Secondary Structure from 1H and 15N NMR" Eur. J. Biochem. 268, 4731-4739 (2001).
PubMed: 11532009

Assembly members:

Assembly members:
chemosensory protein, polymer, 112 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mamestra brassicae Taxonomy ID: 55057 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mamestra brassicae

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
chemosensory protein: EDKYTDKYDNINLDEILANK RLLVAYVNCVMERGKCSPEG KELKEHLQDAIENGCKKCTE NQEKGAYRVIEHLIKNEIEI WRELTAKYDPTGNWRKKYED RAKAAGIVIPEE

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1
coupling_constants
- cc_set_1

Data type	Count
15N chemical shifts	116
1H chemical shifts	724
coupling constants	96

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	chemosensory protein	1

Entities:

Entity 1, chemosensory protein 112 residues - Formula weight is not available

1

GLU

ASP

LYS

TYR

THR

ASP

LYS

TYR

ASP

ASN

2

ILE

ASN

LEU

ASP

GLU

ILE

LEU

ALA

ASN

LYS

3

ARG

LEU

VAL

ALA

TYR

VAL

ASN

CYS

VAL

4

MET

GLU

ARG

GLY

LYS

CYS

SER

PRO

GLU

GLY

5

LYS

GLU

LEU

LYS

GLU

HIS

LEU

GLN

ASP

ALA

6

ILE

GLU

ASN

GLY

CYS

LYS

CYS

THR

GLU

7

ASN

GLN

GLU

LYS

GLY

ALA

TYR

ARG

VAL

ILE

8

GLU

HIS

LEU

ILE

LYS

ASN

GLU

ILE

GLU

ILE

9

TRP

ARG

GLU

LEU

THR

ALA

LYS

TYR

ASP

PRO

10

THR

GLY

ASN

TRP

ARG

LYS

TYR

GLU

ASP

11

ARG

ALA

LYS

ALA

GLY

ILE

VAL

ILE

PRO

12

GLU

Samples:

sample_1: chemosensory protein 2 mM; sodium phosphate buffer 2 mM; H2O 90%; D2O 10%

sample_2: chemosensory protein, [U-15N], 0.5 mM; sodium phosphate buffer 0.5 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.9; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	not available
DQF-COSY	not available	not available	not available
clean-TOCSY	not available	not available	not available
3D 15N-1H-HSQC NOESY	not available	not available	not available
3D 15N-1H-HSQC TOCSY	not available	not available	not available
2D 15N-1H-HSQC	not available	not available	not available

Software:

XWINNMR v2.1 - processing

DIANA v2.8 - structure solution

DYANA v1.5 - structure solution

NMRPipe v1.8 - processing

CNS v1.0 - refinement

turbo frodo vMarch 2001 release a - data analysis

XEASY v1.3.13 - data analysis

Spscan v1.1 - processing

MOLMOL v2.6 - data analysis

NMR spectrometers:

Varian INOVA 750 MHz

PDB	1K19 1KX8 1KX9 1N8U 1N8V
GenBank	AAF19647 AAF19648 AAF19649 AAF19650 AAF19651