BMRB Entry 50936

Name: DksA N88D
Published: 2021-06-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50936

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

DksA N88D

Deposition date:

2021-05-10

Original release date:

2021-06-10

Authors:

Danhart, Eric

Citation:

Citation: Furman, Ran; Danhart, Eric; NandyMazumdar, Monali; Yuan, Chunhua; Foster, Mark; Artsimovitch, Irina. "pH dependence of the stress regulator DksA." PLoS One 10, e0120746-e0120746 (2015).
PubMed: 25799498

Assembly members:

Assembly members:
entity_1, polymer, 154 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pIA1119

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GLQMQEGQNRKTSSLSILAI AGVEPYQEKPGEEYMNEAQL AHFRRILEAWRNQLRDEVDR TVTHMQDEAANFPDPVDRAA QEEEFSLELRDRDRERKLIK KIEKTLKKVEDEDFGYCESC GVEIGIRRLEARPTADLCID CKTLAEIREKQMAG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	296
15N chemical shifts	96
1H chemical shifts	205

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DksA	1

Entities:

Entity 1, DksA 154 residues - Formula weight is not available

1

GLY

LEU

GLN

MET

GLN

GLU

GLY

GLN

ASN

ARG

2

LYS

THR

SER

LEU

SER

ILE

LEU

ALA

ILE

3

ALA

GLY

VAL

GLU

PRO

TYR

GLN

GLU

LYS

PRO

4

GLY

GLU

TYR

MET

ASN

GLU

ALA

GLN

LEU

5

ALA

HIS

PHE

ARG

ILE

LEU

GLU

ALA

TRP

6

ARG

ASN

GLN

LEU

ARG

ASP

GLU

VAL

ASP

ARG

7

THR

VAL

THR

HIS

MET

GLN

ASP

GLU

ALA

8

ASN

PHE

PRO

ASP

PRO

VAL

ASP

ARG

ALA

9

GLN

GLU

PHE

SER

LEU

GLU

LEU

ARG

10

ASP

ARG

ASP

ARG

GLU

ARG

LYS

LEU

ILE

LYS

11

LYS

ILE

GLU

LYS

THR

LEU

LYS

VAL

GLU

12

ASP

GLU

ASP

PHE

GLY

TYR

CYS

GLU

SER

CYS

13

GLY

VAL

GLU

ILE

GLY

ILE

ARG

LEU

GLU

14

ALA

ARG

PRO

THR

ALA

ASP

LEU

CYS

ILE

ASP

15

CYS

LYS

THR

LEU

ALA

GLU

ILE

ARG

GLU

LYS

16

GLN

MET

ALA

GLY

Samples:

sample_1: DksA N88D, [U-13C; U-15N], 0.4 mM; NaCl 200 mM; DTT 1 mM; ZnCl2 50 uM; sodium phosphate 200 mM; sodium azide 0.02%

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
15N-(1H) NOE	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

NMRViewJ - chemical shift assignment

TOPSPIN - collection

NMR spectrometers:

Bruker DRX 600 MHz
Bruker DRX 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks