BMRB Entry 5093
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5093
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
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Citation: Swapna, G.; Shukla, Kamal; Huang, YJ; Ke, H.; Xia, B.; Inouye, Masayori; Montelione, Gaetano. "Resonance assignments for cold-shock protein ribosome-binding factor A (RbfA)
from Escherichia coli" J. Biomol. NMR 21, 389-390 (2001).
PubMed: 11824762
Assembly members:
RbfA, polymer, 108 residues, 12300 Da.
Natural source: Common Name: E. coli. Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a
Entity Sequences (FASTA):
RbfA: MAKEFGRPQRVAQEMQKEIA
LILQREIKDPRLGMMTTVSG
VEMSRDLAYAKVYVTFLNDK
DEDAVKAGIKALQEASGFIR
SLLGKAMRLRIVPELTFFYD
NSLVEGMR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 727 |
| 13C chemical shifts | 442 |
| 15N chemical shifts | 108 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ribosome-binding Factor A | 1 |
Entities:
Entity 1, Ribosome-binding Factor A 108 residues - 12300 Da.
| 1 | MET | ALA | LYS | GLU | PHE | GLY | ARG | PRO | GLN | ARG | ||||
| 2 | VAL | ALA | GLN | GLU | MET | GLN | LYS | GLU | ILE | ALA | ||||
| 3 | LEU | ILE | LEU | GLN | ARG | GLU | ILE | LYS | ASP | PRO | ||||
| 4 | ARG | LEU | GLY | MET | MET | THR | THR | VAL | SER | GLY | ||||
| 5 | VAL | GLU | MET | SER | ARG | ASP | LEU | ALA | TYR | ALA | ||||
| 6 | LYS | VAL | TYR | VAL | THR | PHE | LEU | ASN | ASP | LYS | ||||
| 7 | ASP | GLU | ASP | ALA | VAL | LYS | ALA | GLY | ILE | LYS | ||||
| 8 | ALA | LEU | GLN | GLU | ALA | SER | GLY | PHE | ILE | ARG | ||||
| 9 | SER | LEU | LEU | GLY | LYS | ALA | MET | ARG | LEU | ARG | ||||
| 10 | ILE | VAL | PRO | GLU | LEU | THR | PHE | PHE | TYR | ASP | ||||
| 11 | ASN | SER | LEU | VAL | GLU | GLY | MET | ARG |
Samples:
sample_1: RbfA, [U-13C; U-15N], 1.6 mM
condition_1: pH: 5.05; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| (HA)CA(CO)NH | not available | not available | not available |
| HA(CA)(CO)NH | not available | not available | not available |
| (HA)CANH | not available | not available | not available |
| HA(CA)NH | not available | not available | not available |
| H(CCCO)NH-TOCSY | not available | not available | not available |
| (H)CC(CO)NH-TOCSY | not available | not available | not available |
| HCCH-COSY | not available | not available | not available |
| 13C-edited NOESY | not available | not available | not available |
| 15N-edited NOESY | not available | not available | not available |
Software:
VNMR v6.3b - data processing
SPARKY v3.91 - peak picking
AUTOASSIGN v1.7.8 - automated backbone 1H, 13C and 15N assignments
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 18147 |
| PDB | |
| DBJ | BAB37471 BAE77213 BAG78977 BAH65352 BAI27447 |
| EMBL | CAA31634 CAA32020 CAP77629 CAQ33502 CAQ90639 |
| GB | AAA57970 AAC76201 AAG58303 AAL22157 AAN44675 |
| REF | NP_289743 NP_312075 NP_417636 NP_462198 NP_708968 |
| SP | A1AG72 A6TEI6 A7ZS64 A8A4Y3 A8AQ56 |
| AlphaFold | A1AG72 A6TEI6 A7ZS64 A8A4Y3 A8AQ56 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks