BMRB Entry 50927

Name: RNA73
Published: 2021-05-24

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50927

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

RNA73

Deposition date:

2021-05-05

Original release date:

2021-05-24

Authors:

Liu, Yaping; Keane, Sarah

Citation:

Citation: Liu, Yaping; Kotar, Anita; Hodges, Tracy; Abdallah, Kyrillos; Taleb, Mallak; Bitterman, Brayden; Jaime, Sara; Schaubroeck, Kyle; Mathew, Ethan; Morgenstern, Nick; Lohmeier, Anthony; Page, Jordan; Ratanapanichkich, Matt; Arhin, Grace; Johnson, Breanna; Cherepanov, Stanislav; Moss, Stephen; Zuniga, Gisselle; Tilson, Nicholas; Yeoh, Zoe; Johnson, Bruce; Keane, Sarah. "NMR chemical shift assignments of RNA oligonucleotides to expand the RNA chemical shift database" Biomol. NMR Assign. 15, 479-490 (2021).
PubMed: 34449019

Assembly members:

Assembly members:
entity_1, polymer, 22 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: In vitro transcription Host organism: In vitro

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGGUAGGCUGAGAAGCCUAC CC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	27
1H chemical shifts	116

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RNA73	1

Entities:

Entity 1, RNA73 22 residues - Formula weight is not available

1

G

U

A

G

C

U

G

2

A

G

A

G

C

U

A

C

3

C

Samples:

sample_1: RNA73 400 uM; potassium phosphate 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HMQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRFx Analyst - processing

NMRPipe - processing

NMRViewJ - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz