BMRB Entry 50916

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50916

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Title:
Backbone 1H, 13C and 15N chemical shift assignments of HIV-1 p6.
Deposition date:
2021-04-28
Original release date:
2021-07-29
Authors:
Ramaraju, Bhargavi; Deshmukh, Lalit
Citation:

Citation: Ramaraju, Bhargavi; Nelson, Spencer; Zheng, Wenwei; Ghirlando, Rodolfo; Deshmukh, Lalit. "Quantitative NMR Study of Insulin-Degrading Enzyme Using Amyloid-beta and HIV-1 p6 Elucidates Its Chaperone Activity"  Biochemistry 60, 2519-2523 (2021).
PubMed: 34342986

Assembly members:

Assembly members:
entity_1, polymer, 50 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: HIV-1   Taxonomy ID: 11676   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus HIV-1

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SRPEPTAPPEESFRSGVETT TPPQKQEPIDKELYPLTSLR SLFGNDPSSQ

Data sets:
Data typeCount
13C chemical shifts109
15N chemical shifts36
1H chemical shifts36

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HIV-1 p61

Entities:

Entity 1, HIV-1 p6 50 residues - Formula weight is not available

1   SERARGPROGLUPROTHRALAPROPROGLU
2   GLUSERPHEARGSERGLYVALGLUTHRTHR
3   THRPROPROGLNLYSGLNGLUPROILEASP
4   LYSGLULEUTYRPROLEUTHRSERLEUARG
5   SERLEUPHEGLYASNASPPROSERSERGLN

Samples:

sample_1: HIV-1 p6, [U-100% 13C; U-100% 15N], 0.75 mM; TRIS 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.2; pressure: 1 atm; temperature: 275.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker AVANCE NEO 600 MHz

Related Database Links:

UNP P04591
AlphaFold P04591

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks