BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 50915

Title: WT nsp1   PubMed: 34874953

Deposition date: 2021-04-28 Original release date: 2021-11-04

Authors: Agback, Tatiana; Dominguez, Francisco; Frolov, Ilya; Frolova, Elena; Agback, Peter

Citation: Agback, Tatiana; Dominguez, Francisco; Frolov, Ilya; Frolova, Elena; Agback, Peter. "1H, 13C and 15N resonance assignment of the SARS-CoV-2 full-length nsp1 protein and its mutants reveals its unique secondary structure features in solution"  PLoS One 16, e0251834-e0251834 (2021).

Assembly members:
entity_1, polymer, 180 residues, Formula weight is not available

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pE-SUMOpro-3

Entity Sequences (FASTA):
entity_1: MESLVPGFNEKTHVQLSLPV LQVRDVLVRGFGDSVEEVLS EARQHLKDGTCGLVEVEKGV LPQLEQPYVFIKRSDARTAP HGHVMVELVAELEGIQYGRS GETLGVLVPHVGEIPVAYRK VLLRKNGNKGAGGHSYGADL KSFDLGDELGTDPYEDFQEN WNTKHSSGVTRELMRELNGG

Data sets:
Data typeCount
13C chemical shifts909
15N chemical shifts322
1H chemical shifts619

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1nsp11

Entities:

Entity 1, nsp1 180 residues - Formula weight is not available

1   METGLUSERLEUVALPROGLYPHEASNGLU
2   LYSTHRHISVALGLNLEUSERLEUPROVAL
3   LEUGLNVALARGASPVALLEUVALARGGLY
4   PHEGLYASPSERVALGLUGLUVALLEUSER
5   GLUALAARGGLNHISLEULYSASPGLYTHR
6   CYSGLYLEUVALGLUVALGLULYSGLYVAL
7   LEUPROGLNLEUGLUGLNPROTYRVALPHE
8   ILELYSARGSERASPALAARGTHRALAPRO
9   HISGLYHISVALMETVALGLULEUVALALA
10   GLULEUGLUGLYILEGLNTYRGLYARGSER
11   GLYGLUTHRLEUGLYVALLEUVALPROHIS
12   VALGLYGLUILEPROVALALATYRARGLYS
13   VALLEULEUARGLYSASNGLYASNLYSGLY
14   ALAGLYGLYHISSERTYRGLYALAASPLEU
15   LYSSERPHEASPLEUGLYASPGLULEUGLY
16   THRASPPROTYRGLUASPPHEGLNGLUASN
17   TRPASNTHRLYSHISSERSERGLYVALTHR
18   ARGGLULEUMETARGGLULEUASNGLYGLY

Samples:

sample_1: wt nsp1, [U-100% 13C; U-100% 15N], 0.4 mM; KCl 100 mM; NaN3 1 mM; DSS 0.1 mM

sample_conditions_1: ionic strength: 0.101 M; pH: 7.5; pressure: 1 atm; temperature: 308 K

sample_conditions_2: ionic strength: 0.101 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5 - collection

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts