BMRB Entry 50914

Name: Backbone (NH, CA, CB) chemical shifts of free ERH-2 from C.elegans
Published: 2021-08-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50914

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone (NH, CA, CB) chemical shifts of free ERH-2 from C.elegans

Deposition date:

2021-04-28

Original release date:

2021-08-28

Authors:

Perez-Borrajero, Cecilia; Hennig, Janosch

Citation:

Citation: Perez-Borrajero, Cecilia; Podvalnaya, Nadezda; Holleis, Kay; Lichtenberger, Raffael; Karaulanov, Emil; Simon, Bernd; Basquin, Jerome; Hennig, Janosch; Ketting, Rene; Falk, Sebastian. "Structural basis of PETISCO complex assembly during piRNA biogenesis in C. elegans" Genes Dev. 35, 1304-1323 (2021).
PubMed: 34413138

Assembly members:

Assembly members:
entity_1, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source: Common Name: C. elegans Taxonomy ID: 6239 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHMASMSTSSHTVLLIQTSP RLDSRTWGDYESVTDALDAL CKMFEDFLSKKSAAPVTYDV SQVYEFLDKLSDVSMMIFNR ETGQYIGRTRAWIKQQVYEM MRGRCQHPEGGEKVIVGY

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	191
15N chemical shifts	99
1H chemical shifts	99

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ERH-2, chain 1	1
2	ERH-2, chain 2	1

Entities:

Entity 1, ERH-2, chain 1 118 residues - Formula weight is not available

1

GLY

HIS

MET

ALA

SER

MET

SER

THR

SER

2

HIS

THR

VAL

LEU

ILE

GLN

THR

SER

PRO

3

ARG

LEU

ASP

SER

ARG

THR

TRP

GLY

ASP

TYR

4

GLU

SER

VAL

THR

ASP

ALA

LEU

ASP

ALA

LEU

5

CYS

LYS

MET

PHE

GLU

ASP

PHE

LEU

SER

LYS

6

LYS

SER

ALA

PRO

VAL

THR

TYR

ASP

VAL

7

SER

GLN

VAL

TYR

GLU

PHE

LEU

ASP

LYS

LEU

8

SER

ASP

VAL

SER

MET

ILE

PHE

ASN

ARG

9

GLU

THR

GLY

GLN

TYR

ILE

GLY

ARG

THR

ARG

10

ALA

TRP

ILE

LYS

GLN

VAL

TYR

GLU

MET

11

MET

ARG

GLY

ARG

CYS

GLN

HIS

PRO

GLU

GLY

12

GLY

GLU

LYS

VAL

ILE

VAL

GLY

TYR

Samples:

sample_1: ERH-2, [U-100% 13C; U-100% 15N; U-80% 2H], 600 ± 50 uM

sample_conditions_1: ionic strength: 0.25 M; pH: 7.42; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.9.1.7 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks