BMRB Entry 50910

Title:
Rules for designing protein fold switches and their implications for the folding code
Deposition date:
2021-04-22
Original release date:
2023-01-03
Authors:
He, Yanan; Chen, Yingwei; Ruan, Biao; Choi, Eun; Chen, Yihong; Motabar, Dana; Solomon, Tsega; Simmerman, Richard; Kauffman, Thomas; Gallagher, D,Travis; Bryan, Philip; Orban, John
Citation:

Citation: He, Yanan; Chen, Yingwei; Ruan, Biao; Choi, Eun; Chen, Yihong; Motabar, Dana; Solomon, Tsega; Simmerman, Richard; Kauffman, Thomas; Gallagher, D,Travis; Bryan, Philip; Orban, John. "Design and characterization of a protein fold switching network"  Nat. Commun. 14, 431-431 (2023).
PubMed: 36702827

Assembly members:

Assembly members:
entity_1, polymer, 56 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pA-YRGL

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts161
15N chemical shifts55
1H chemical shifts116

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1B11

Entities:

Entity 1, B1 56 residues - Formula weight is not available

1   ALATHRPHELYSLEUVALLEUASNGLYLYS
2   THRLEULYSGLYGLUTHRTHRTHRGLUALA
3   VALASPALAALATHRALALEULYSASNPHE
4   GLYALATYRALAGLNASPVALGLYVALASP
5   GLYALATRPTHRTYRASPASPALATHRLYS
6   THRPHETHRVALGLYGLU

Samples:

sample_1: B1, [U-13C; U-15N], 0.3 mM; D2O 5 V/V%; potassium phosphate 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3DHNCACBsample_1isotropicsample_conditions_1
3DCBCACONHsample_1isotropicsample_conditions_1
3DHNHAsample_1isotropicsample_conditions_1
3DHNCOsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.61 - collection

NMRFAM-SPARKY - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks