BMRB Entry 50908

Name: human CSNAP
Published: 2021-08-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50908

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

human CSNAP

Deposition date:

2021-04-22

Original release date:

2021-08-16

Authors:

Ruidiaz, Sarah; Dreier, Jesper; Hartmann-Petersen, Rasmus; Kragelund, Birthe

Citation:

Citation: Ruidiaz, Sarah; Dreier, Jesper; Hartmann-Petersen, Rasmus; Kragelund, Birthe. "The disordered PCI-binding human proteins CSNAP and DSS1 have diverged in structure and function" Protein Sci. 30, 2069-2082 (2021).
PubMed: 34272906

Assembly members:

Assembly members:
entity_1, polymer, 58 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pD454-GST

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GMKPAVDEMFPEGAGPYVDL DEAGGSTGLLMDLAANEKAV HADFFNDFEDLFDDDDIQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	166
15N chemical shifts	53
1H chemical shifts	53

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CSNAP	1

Entities:

Entity 1, CSNAP 58 residues - Formula weight is not available

1

GLY

MET

LYS

PRO

ALA

VAL

ASP

GLU

MET

PHE

2

PRO

GLU

GLY

ALA

GLY

PRO

TYR

VAL

ASP

LEU

3

ASP

GLU

ALA

GLY

SER

THR

GLY

LEU

4

MET

ASP

LEU

ALA

ASN

GLU

LYS

ALA

VAL

5

HIS

ALA

ASP

PHE

ASN

ASP

PHE

GLU

ASP

6

LEU

PHE

ASP

ILE

GLN

Samples:

sample_1: CSNAP, [U-100% 13C; U-100% 15N], 210 uM; sodium phosphate 20 mM; sodium chloride 150 mM; DSS 1%; sodium azide 1%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.4; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)NNH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

Agilent DD2 800 MHz
Bruker AVANCE III 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks