BMRB Entry 50906

Name: Chemical shifts of human Dss1
Published: 2021-08-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50906

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Chemical shifts of human Dss1

Deposition date:

2021-04-22

Original release date:

2021-08-16

Authors:

Ruidiaz, Sarah; Dreier, Jesper; Hartmann-Petersen, Rasmus; Kragelund, Birthe

Citation:

Citation: Ruidiaz, Sarah; Dreier, Jesper; Hartmann-Petersen, Rasmus; Kragelund, Birthe. "The disordered PCI-binding human proteins CSNAP and DSS1 have diverged in structure and function" Protein Sci. 30, 2069-2082 (2021).
PubMed: 34272906

Assembly members:

Assembly members:
entity_1, polymer, 71 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pD454-GST

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GMSEKKQPVDLGLLEEDDEF EEFPAEDWAGLDEDEDAHVW EDNWDDDNVEDDFSNQLRAE LEKHGYKMETS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	207
15N chemical shifts	67
1H chemical shifts	67

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Dss1	1

Entities:

Entity 1, Dss1 71 residues - Formula weight is not available

Non-native N-terminal Glycine

1

GLY

MET

SER

GLU

LYS

GLN

PRO

VAL

ASP

2

LEU

GLY

LEU

GLU

ASP

GLU

PHE

3

GLU

PHE

PRO

ALA

GLU

ASP

TRP

ALA

GLY

4

LEU

ASP

GLU

ASP

GLU

ASP

ALA

HIS

VAL

TRP

5

GLU

ASP

ASN

TRP

ASP

ASN

VAL

GLU

6

ASP

PHE

SER

ASN

GLN

LEU

ARG

ALA

GLU

7

LEU

GLU

LYS

HIS

GLY

TYR

LYS

MET

GLU

THR

8

SER

Samples:

sample_1: DSS1, [U-100% 13C; U-100% 15N], 140 uM; sodium phosphate 20 mM; sodium chloride 150 mM; DSS 1%; sodium azide 1%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.4; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)NNH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks