BMRB Entry 50905

Title:
TCPTP residues 303-387 RK variant
Deposition date:
2021-04-20
Original release date:
2021-11-10
Authors:
Singh, Jai; Li, Yang; Peti, Wolfgang; Meng, Tzu-Ching; Page, Rebecca
Citation:

Citation: Singh, Jai Prakash; Li, Yang; Chen, Yi-Yun; Hsu, Shang-Te Danny; Page, Rebecca; Peti, Wolfgang; Meng, Tzu-Ching. "The catalytic activity of TCPTP is auto-regulated by its intrinsically disordered tail and activated by Integrin alpha-1"  Nat. Commun. 13, 94-94 (2022).
PubMed: 35013194

Assembly members:

Assembly members:
entity_1, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTHMT

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts149
15N chemical shifts77
1H chemical shifts77

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TCPTP residues 303-387 RK variant1

Entities:

Entity 1, TCPTP residues 303-387 RK variant 90 residues - Formula weight is not available

The first five amino acids GHMGS are from cloning vector, TCPTP sequence starts after the GHMGS.

1   GLYHISMETGLYSERHISSERPROASNLYS
2   ILEMETTHRGLULYSTYRASNGLYASNARG
3   ILEGLYLEUGLUGLUGLULYSLEUTHRGLY
4   ASPARGCYSTHRGLYLEUSERSERLYSMET
5   GLNASPTHRMETGLUGLUASNSERGLUSER
6   ALALEUARGLYSARGILEARGGLUASPARG
7   LYSALATHRTHRALAGLNLYSVALGLNGLN
8   METLYSGLNARGLEUARGLYSARGLYSARG
9   LYSARGLYSARGPROARGLEUTHRASPTHR

Samples:

sample_1: TCPTP residues 303-387 RK variant, [U-100% 13C; U-100% 15N], 600 uM; HEPES 20 mM; sodium chloride 150 mM; TCEP 0.5 mM

sample_conditions_1: ionic strength: 170 mM; pH: 6.8; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection, processing

CARA - chemical shift assignment

CcpNMR - data analysis

NMR spectrometers:

  • Bruker AVANCE NEO 800 MHz

Related Database Links:

UNP P17706-2
AlphaFold Q96HR2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks