BMRB Entry 50905

Name: TCPTP residues 303-387 RK variant
Published: 2021-11-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50905

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

TCPTP residues 303-387 RK variant

Deposition date:

2021-04-20

Original release date:

2021-11-10

Authors:

Singh, Jai; Li, Yang; Peti, Wolfgang; Meng, Tzu-Ching; Page, Rebecca

Citation:

Citation: Singh, Jai Prakash; Li, Yang; Chen, Yi-Yun; Hsu, Shang-Te Danny; Page, Rebecca; Peti, Wolfgang; Meng, Tzu-Ching. "The catalytic activity of TCPTP is auto-regulated by its intrinsically disordered tail and activated by Integrin alpha-1" Nat. Commun. 13, 94-94 (2022).
PubMed: 35013194

Assembly members:

Assembly members:
entity_1, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTHMT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHMGSHSPNKIMTEKYNGNR IGLEEEKLTGDRCTGLSSKM QDTMEENSESALRKRIREDR KATTAQKVQQMKQRLRKRKR KRKRPRLTDT

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	149
15N chemical shifts	77
1H chemical shifts	77

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TCPTP residues 303-387 RK variant	1

Entities:

Entity 1, TCPTP residues 303-387 RK variant 90 residues - Formula weight is not available

The first five amino acids GHMGS are from cloning vector, TCPTP sequence starts after the GHMGS.

1	GLY	HIS	MET	GLY	SER	HIS	SER	PRO	ASN	LYS
2	ILE	MET	THR	GLU	LYS	TYR	ASN	GLY	ASN	ARG
3	ILE	GLY	LEU	GLU	GLU	GLU	LYS	LEU	THR	GLY
4	ASP	ARG	CYS	THR	GLY	LEU	SER	SER	LYS	MET
5	GLN	ASP	THR	MET	GLU	GLU	ASN	SER	GLU	SER
6	ALA	LEU	ARG	LYS	ARG	ILE	ARG	GLU	ASP	ARG
7	LYS	ALA	THR	THR	ALA	GLN	LYS	VAL	GLN	GLN
8	MET	LYS	GLN	ARG	LEU	ARG	LYS	ARG	LYS	ARG
9	LYS	ARG	LYS	ARG	PRO	ARG	LEU	THR	ASP	THR

Samples:

sample_1: TCPTP residues 303-387 RK variant, [U-100% 13C; U-100% 15N], 600 uM; HEPES 20 mM; sodium chloride 150 mM; TCEP 0.5 mM

sample_conditions_1: ionic strength: 170 mM; pH: 6.8; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection, processing

CARA - chemical shift assignment

CcpNMR - data analysis

NMR spectrometers:

Bruker AVANCE NEO 800 MHz

UNP	P17706-2
AlphaFold	Q96HR2

1	GLY	HIS	MET	GLY	SER	HIS	SER	PRO	ASN	LYS
2	ILE	MET	THR	GLU	LYS	TYR	ASN	GLY	ASN	ARG
3	ILE	GLY	LEU	GLU	GLU	GLU	LYS	LEU	THR	GLY
4	ASP	ARG	CYS	THR	GLY	LEU	SER	SER	LYS	MET
5	GLN	ASP	THR	MET	GLU	GLU	ASN	SER	GLU	SER
6	ALA	LEU	ARG	LYS	ARG	ILE	ARG	GLU	ASP	ARG
7	LYS	ALA	THR	THR	ALA	GLN	LYS	VAL	GLN	GLN
8	MET	LYS	GLN	ARG	LEU	ARG	LYS	ARG	LYS	ARG
9	LYS	ARG	LYS	ARG	PRO	ARG	LEU	THR	ASP	THR

1	GLY	HIS	MET	GLY	SER	HIS	SER	PRO	ASN	LYS
2	ILE	MET	THR	GLU	LYS	TYR	ASN	GLY	ASN	ARG
3	ILE	GLY	LEU	GLU	GLU	GLU	LYS	LEU	THR	GLY
4	ASP	ARG	CYS	THR	GLY	LEU	SER	SER	LYS	MET
5	GLN	ASP	THR	MET	GLU	GLU	ASN	SER	GLU	SER
6	ALA	LEU	ARG	LYS	ARG	ILE	ARG	GLU	ASP	ARG
7	LYS	ALA	THR	THR	ALA	GLN	LYS	VAL	GLN	GLN
8	MET	LYS	GLN	ARG	LEU	ARG	LYS	ARG	LYS	ARG
9	LYS	ARG	LYS	ARG	PRO	ARG	LEU	THR	ASP	THR