BMRB Entry 50900

Name: A peptide with high affinity for murine double minute2
Published: 2021-04-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50900

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

A peptide with high affinity for murine double minute2

Deposition date:

2021-04-18

Original release date:

2021-04-30

Authors:

Fan, Shihui

Citation:

Citation: Zha, Jun; Li, Jinjing; Fan, Shihui; Duan, Zengping; Zhao, Yibing; Wu, Chuanliu. "An evolution-inspired strategy to design disulfide-rich peptides tolerant to extensive sequence manipulation" Chem. Sci. 12, 11464-11472 (2021).
PubMed: 34567500

Assembly members:

Assembly members:
entity_1, polymer, 26 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GCEKATESESPCPCIFPELC PWIVCC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	71
15N chemical shifts	18
1H chemical shifts	150

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	16x_MDM2	1

Entities:

Entity 1, 16x_MDM2 26 residues - Formula weight is not available

1

GLY

CYS

GLU

LYS

ALA

THR

GLU

SER

GLU

SER

2

PRO

CYS

PRO

CYS

ILE

PHE

PRO

GLU

LEU

CYS

3

PRO

TRP

ILE

VAL

CYS

Samples:

sample_1: 16x_MDM2 0.8 mM

sample_conditions_1: ionic strength: 0 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

TOPSPIN - collection

NMRPipe - processing

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks