BMRB Entry 5090

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5090

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Title:
Letter to the Editor: 1H(N), 15N, 13CO, 13C[agr], 13C[bgr] Assignment and Secondary Structure of a 20 kDa [agr]-L-fucosidase from Pea using TROSY
Deposition date:
2001-07-19
Original release date:
2002-08-22
Authors:
Codina, Anna; Gairi, Margarida; Tarrago, Teresa; Viguera, Ana Rosa; Feliz, Miguel; Ludevid, Dolors; Giralt, Ernest
Citation:

Citation: Codina, Anna; Gairi, Margarida; Tarrago, Teresa; Viguera, Ana Rosa; Feliz, Miguel; Ludevid, Dolors; Giralt, Ernest. "Letter to the Editor: 1H(N), 15N, 13CO, 13C[agr], 13C[bgr] Assignment and Secondary Structure of a 20 kDa [agr]-L-fucosidase from Pea using TROSY"  J. Biomol. NMR 22, 295-296 (2002).
PubMed: 11991358

Assembly members:

Assembly members:
pea alfa-L-fucosidase, polymer, 195 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pea   Taxonomy ID: 3888   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Pisum sativum

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
pea alfa-L-fucosidase: GSHMEDVEQVLDINGNPIFP GVQYFILPAIRGPAGGGVRL GRTGDLTCPVTVLQDRQEVK YGLPVKFVIPEISPGIIFTG TPIEIEYAKKPNCAKSSKWL VFVDNVIQKACVGIGGPENY PGVQTLSGLFKIEKHESGFG YKLGFCVKGSPTCLDVGRFD NDEDGRRLNLTEHESFQVVF IQAEANDAEFIKSVV

Data sets:
Data typeCount
1H chemical shifts156
13C chemical shifts505
15N chemical shifts156

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1alfa-L-fucosidase1

Entities:

Entity 1, alfa-L-fucosidase 195 residues - Formula weight is not available

1   GLYSERHISMETGLUASPVALGLUGLNVAL
2   LEUASPILEASNGLYASNPROILEPHEPRO
3   GLYVALGLNTYRPHEILELEUPROALAILE
4   ARGGLYPROALAGLYGLYGLYVALARGLEU
5   GLYARGTHRGLYASPLEUTHRCYSPROVAL
6   THRVALLEUGLNASPARGGLNGLUVALLYS
7   TYRGLYLEUPROVALLYSPHEVALILEPRO
8   GLUILESERPROGLYILEILEPHETHRGLY
9   THRPROILEGLUILEGLUTYRALALYSLYS
10   PROASNCYSALALYSSERSERLYSTRPLEU
11   VALPHEVALASPASNVALILEGLNLYSALA
12   CYSVALGLYILEGLYGLYPROGLUASNTYR
13   PROGLYVALGLNTHRLEUSERGLYLEUPHE
14   LYSILEGLULYSHISGLUSERGLYPHEGLY
15   TYRLYSLEUGLYPHECYSVALLYSGLYSER
16   PROTHRCYSLEUASPVALGLYARGPHEASP
17   ASNASPGLUASPGLYARGARGLEUASNLEU
18   THRGLUHISGLUSERPHEGLNVALVALPHE
19   ILEGLNALAGLUALAASNASPALAGLUPHE
20   ILELYSSERVALVAL

Samples:

sample_1: pea alfa-L-fucosidase, [U-99% 13C; U-99% 15N; U-85% 2H], 0.8 – 1.0 mM

cond_1: pH: 7.7; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

EMBL CAA57931
SP Q41015
AlphaFold Q41015

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks