BMRB Entry 50880

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50880

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
CREB 285 to 315 apo
Deposition date:
2021-04-08
Original release date:
2024-08-28
Authors:
Kelly, Conor Francis; Redfield, Christina; Shammas, Sarah
Citation:

Citation: Kelly, Conor Francis; Kuravsky, Mikhail; Redfield, Christina; Shammas, Sarah. "The transition state for coupled folding and binding of a disordered DNA binding domain resembles the unbound state"  Nucleic Acids Res. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 32 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHSG299

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GKREVRLMKNREAARESRRK KKEYVKSLENRV

Data sets:
Data typeCount
13C chemical shifts139
15N chemical shifts30
1H chemical shifts230

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CREB 285-3151

Entities:

Entity 1, CREB 285-315 32 residues - Formula weight is not available

1   GLYLYSARGGLUVALARGLEUMETLYSASN
2   ARGGLUALAALAARGGLUSERARGARGLYS
3   LYSLYSGLUTYRVALLYSSERLEUGLUASN
4   ARGVAL

Samples:

sample_1: CREB 285-315, [U-98% 13C; U-98% 15N], 400 uM; Magnesium chloride 10 mM; DSS 10 uM; sodium azide 1 mM; sodium chloride 150 mM; TRIS, [U-2H], 10 mM

sample_conditions_1: ionic strength: 0.185 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HMQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.2 - collection

NMRPipe v9.8 - data analysis

ANALYSIS v2.4.2 - chemical shift assignment

istHMS vv211_64b - processing

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks