BMRB Entry 50866

Name: CITED2-HIF-1alpha fusion peptide
Published: 2021-07-24

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50866

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

CITED2-HIF-1alpha fusion peptide

Deposition date:

2021-03-31

Original release date:

2021-07-24

Authors:

Appling, Francis; Berlow, Rebecca; Stanfield, Robyn; Dyson, H Jane; Wright, Peter

Citation:

Citation: Appling, Francis; Berlow, Rebecca; Stanfield, Robyn; Dyson, H Jane; Wright, Peter. "The molecular basis of allostery in a facilitated dissociation process" Structure 29, 1327-1338 (2021).
PubMed: 34520739

Assembly members:

Assembly members:
entity_1, polymer, 67 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSHMSNVIDTDFIDEEVLMS LVIEMGLDRIKELPELTSYD CEVNAPIQGSRNLLQGEELL RALDQVN

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	188
15N chemical shifts	61
1H chemical shifts	61

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CITED2-HIF-1alpha fusion peptide	1

Entities:

Entity 1, CITED2-HIF-1alpha fusion peptide 67 residues - Formula weight is not available

1

GLY

SER

HIS

MET

SER

ASN

VAL

ILE

ASP

THR

2

ASP

PHE

ILE

ASP

GLU

VAL

LEU

MET

SER

3

LEU

VAL

ILE

GLU

MET

GLY

LEU

ASP

ARG

ILE

4

LYS

GLU

LEU

PRO

GLU

LEU

THR

SER

TYR

ASP

5

CYS

GLU

VAL

ASN

ALA

PRO

ILE

GLN

GLY

SER

6

ARG

ASN

LEU

GLN

GLY

GLU

LEU

7

ARG

ALA

LEU

ASP

GLN

VAL

ASN

Samples:

sample_1: CITED2-HIF-1alpha fusion peptide, [U-100% 13C; U-100% 15N], 100 uM; NaCl 50 mM; DTT 2 mM; TRIS 20 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D best-HNCO	sample_1	isotropic	sample_conditions_1
3D best-HNCA	sample_1	isotropic	sample_conditions_1
3D best-HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D best-HNCACB	sample_1	isotropic	sample_conditions_1
3D best-HN(CO)CACB	sample_1	isotropic	sample_conditions_1

Software:

NMRbox - data analysis, processing

GNU Octave - data analysis

NMRFAM-SPARKY - data analysis

NMRPipe - processing

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks