BMRB Entry 50866

Title:
CITED2-HIF-1alpha fusion peptide
Deposition date:
2021-03-31
Original release date:
2021-07-24
Authors:
Appling, Francis; Berlow, Rebecca; Stanfield, Robyn; Dyson, H Jane; Wright, Peter
Citation:

Citation: Appling, Francis; Berlow, Rebecca; Stanfield, Robyn; Dyson, H Jane; Wright, Peter. "The molecular basis of allostery in a facilitated dissociation process"  Structure 29, 1327-1338 (2021).
PubMed: 34520739

Assembly members:

Assembly members:
entity_1, polymer, 67 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts188
15N chemical shifts61
1H chemical shifts61

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CITED2-HIF-1alpha fusion peptide1

Entities:

Entity 1, CITED2-HIF-1alpha fusion peptide 67 residues - Formula weight is not available

1   GLYSERHISMETSERASNVALILEASPTHR
2   ASPPHEILEASPGLUGLUVALLEUMETSER
3   LEUVALILEGLUMETGLYLEUASPARGILE
4   LYSGLULEUPROGLULEUTHRSERTYRASP
5   CYSGLUVALASNALAPROILEGLNGLYSER
6   ARGASNLEULEUGLNGLYGLUGLULEULEU
7   ARGALALEUASPGLNVALASN

Samples:

sample_1: CITED2-HIF-1alpha fusion peptide, [U-100% 13C; U-100% 15N], 100 uM; NaCl 50 mM; DTT 2 mM; TRIS 20 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D best-HNCOsample_1isotropicsample_conditions_1
3D best-HNCAsample_1isotropicsample_conditions_1
3D best-HN(CO)CAsample_1isotropicsample_conditions_1
3D best-HNCACBsample_1isotropicsample_conditions_1
3D best-HN(CO)CACBsample_1isotropicsample_conditions_1

Software:

NMRbox - data analysis, processing

GNU Octave - data analysis

NMRFAM-SPARKY - data analysis

NMRPipe - processing

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks