BMRB Entry 50861

Name: Tim10
Published: 2021-04-22

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50861

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Tim10

Deposition date:

2021-03-27

Original release date:

2021-04-22

Authors:

Weinhaupl, Katharina; Wang, Yong; Hessel, Audrey; Brennich, Martha; Lindorff-Larsen, Kresten; Schanda, Paul

Citation:

Citation: Weinhaupl, Katharina; Wang, Yong; Hessel, Audrey; Brennich, Martha; Lindorff-Larsen, Kresten; Schanda, Paul. "Architecture and assembly dynamics of the essential mitochondrial chaperone complex TIM9*10*12" Structure 29, 1065-1073 (2021).
PubMed: 33974880

Assembly members:

Assembly members:
entity_1, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETDuet

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSFLGFGGGQPQLSSQQKIQ AAEAELDLVTDMFNKLVNNC YKKCINTSYSEGELNKNESS CLDRCVAKYFETNVQVGENM QKMGQSFNAAGKF

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	142
15N chemical shifts	61
1H chemical shifts	61

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Tim10	1

Entities:

Entity 1, Tim10 93 residues - Formula weight is not available

1

GLY

SER

PHE

LEU

GLY

PHE

GLY

GLN

2

PRO

GLN

LEU

SER

GLN

LYS

ILE

GLN

3

ALA

GLU

ALA

GLU

LEU

ASP

LEU

VAL

THR

4

ASP

MET

PHE

ASN

LYS

LEU

VAL

ASN

CYS

5

TYR

LYS

CYS

ILE

ASN

THR

SER

TYR

SER

6

GLU

GLY

GLU

LEU

ASN

LYS

ASN

GLU

SER

7

CYS

LEU

ASP

ARG

CYS

VAL

ALA

LYS

TYR

PHE

8

GLU

THR

ASN

VAL

GLN

VAL

GLY

GLU

ASN

MET

9

GLN

LYS

MET

GLY

GLN

SER

PHE

ASN

ALA

10

GLY

LYS

PHE

Samples:

sample_1: Tim10, [U-13C; U-15N], 0.4 mM; MES 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

ANALYSIS - chemical shift assignment

NMRPipe - processing

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks