BMRB Entry 50860
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50860
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NMR-STAR v3 text file.
XML gzip file.
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Citation: Weinhaupl, Katharina; Wang, Yong; Hessel, Audrey; Brennich, Martha; Lindorff-Larsen, Kresten; Schanda, Paul. "Architecture and assembly dynamics of the essential mitochondrial chaperone complex TIM9*10*12" Structure 29, 1065-1073 (2021).
PubMed: 33974880
Assembly members:
entity_1, polymer, 87 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETDuet
Entity Sequences (FASTA):
entity_1: MDALNSKEQQEFQKVVEQKQ
MKDFMRLYSNLVERCFTDCV
NDFTTSKLTNKEQTCIMKCS
EKFLKHSERVGQRFQEQNAA
LGQGLGR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 217 |
| 15N chemical shifts | 75 |
| 1H chemical shifts | 75 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tim9 reduced | 1 |
Entities:
Entity 1, Tim9 reduced 87 residues - Formula weight is not available
| 1 | MET | ASP | ALA | LEU | ASN | SER | LYS | GLU | GLN | GLN | ||||
| 2 | GLU | PHE | GLN | LYS | VAL | VAL | GLU | GLN | LYS | GLN | ||||
| 3 | MET | LYS | ASP | PHE | MET | ARG | LEU | TYR | SER | ASN | ||||
| 4 | LEU | VAL | GLU | ARG | CYS | PHE | THR | ASP | CYS | VAL | ||||
| 5 | ASN | ASP | PHE | THR | THR | SER | LYS | LEU | THR | ASN | ||||
| 6 | LYS | GLU | GLN | THR | CYS | ILE | MET | LYS | CYS | SER | ||||
| 7 | GLU | LYS | PHE | LEU | LYS | HIS | SER | GLU | ARG | VAL | ||||
| 8 | GLY | GLN | ARG | PHE | GLN | GLU | GLN | ASN | ALA | ALA | ||||
| 9 | LEU | GLY | GLN | GLY | LEU | GLY | ARG |
Samples:
sample_1: Tim9, [U-13C; U-15N], 0.4 mM; MES 20 mM; sodium chloride 50 mM; TCEP 10 mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
ANALYSIS - chemical shift assignment
NMRPipe - processing
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks