BMRB Entry 50858

Name: TIM91012
Published: 2021-04-22

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50858

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

TIM91012

Deposition date:

2021-03-26

Original release date:

2021-04-22

Authors:

Weinhaupl, Katharina; Wang, Yong; Hessel, Audrey; Brennich, Martha; Lindorff-Larsen, Kresten; Schanda, Paul

Citation:

Citation: Weinhaupl, Katharina; Wang, Yong; Hessel, Audrey; Brennich, Martha; Lindorff-Larsen, Kresten; Schanda, Paul. "Architecture and assembly dynamics of the essential mitochondrial chaperone complex TIM9*10*12" Structure 29, 1065-1073 (2021).
PubMed: 33974880

Assembly members:

Assembly members:
entity_1, polymer, 87 residues, Formula weight is not available
entity_2, polymer, 93 residues, Formula weight is not available
entity_3, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETDuet

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MDALNSKEQQEFQKVVEQKQ MKDFMRLYSNLVERCFTDCV NDFTTSKLTNKEQTCIMKCS EKFLKHSERVGQRFQEQNAA LGQGLGR
entity_2: GSFLGFGGGQPQLSSQQKIQ AAEAELDLVTDMFNKLVNNC YKKCINTSYSEGELNKNESS CLDRCVAKYFETNVQVGENM QKMGQSFNAAGKF
entity_3: GSFFLNSLRGNQEVSQEKLD VAGVQFDAMASTFNNILSTC LEKCIPHEGFGEPDLTKGEQ ACIDRCVAKMHYSNRLIGGF VQTRGFGPENQLRHYSRFVA KEIADDSKK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	45
15N chemical shifts	28
1H chemical shifts	27

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Tim9	1
2	Tim10	2
3	Tim12	3

Entities:

Entity 1, Tim9 87 residues - Formula weight is not available

1

MET

ASP

ALA

LEU

ASN

SER

LYS

GLU

GLN

2

GLU

PHE

GLN

LYS

VAL

GLU

GLN

LYS

GLN

3

MET

LYS

ASP

PHE

MET

ARG

LEU

TYR

SER

ASN

4

LEU

VAL

GLU

ARG

CYS

PHE

THR

ASP

CYS

VAL

5

ASN

ASP

PHE

THR

SER

LYS

LEU

THR

ASN

6

LYS

GLU

GLN

THR

CYS

ILE

MET

LYS

CYS

SER

7

GLU

LYS

PHE

LEU

LYS

HIS

SER

GLU

ARG

VAL

8

GLY

GLN

ARG

PHE

GLN

GLU

GLN

ASN

ALA

9

LEU

GLY

GLN

GLY

LEU

GLY

ARG

Entity 2, Tim10 93 residues - Formula weight is not available

1

GLY

SER

PHE

LEU

GLY

PHE

GLY

GLN

2

PRO

GLN

LEU

SER

GLN

LYS

ILE

GLN

3

ALA

GLU

ALA

GLU

LEU

ASP

LEU

VAL

THR

4

ASP

MET

PHE

ASN

LYS

LEU

VAL

ASN

CYS

5

TYR

LYS

CYS

ILE

ASN

THR

SER

TYR

SER

6

GLU

GLY

GLU

LEU

ASN

LYS

ASN

GLU

SER

7

CYS

LEU

ASP

ARG

CYS

VAL

ALA

LYS

TYR

PHE

8

GLU

THR

ASN

VAL

GLN

VAL

GLY

GLU

ASN

MET

9

GLN

LYS

MET

GLY

GLN

SER

PHE

ASN

ALA

10

GLY

LYS

PHE

Entity 3, Tim12 109 residues - Formula weight is not available

1

GLY

SER

PHE

LEU

ASN

SER

LEU

ARG

GLY

2

ASN

GLN

GLU

VAL

SER

GLN

GLU

LYS

LEU

ASP

3

VAL

ALA

GLY

VAL

GLN

PHE

ASP

ALA

MET

ALA

4

SER

THR

PHE

ASN

ILE

LEU

SER

THR

CYS

5

LEU

GLU

LYS

CYS

ILE

PRO

HIS

GLU

GLY

PHE

6

GLY

GLU

PRO

ASP

LEU

THR

LYS

GLY

GLU

GLN

7

ALA

CYS

ILE

ASP

ARG

CYS

VAL

ALA

LYS

MET

8

HIS

TYR

SER

ASN

ARG

LEU

ILE

GLY

PHE

9

VAL

GLN

THR

ARG

GLY

PHE

GLY

PRO

GLU

ASN

10

GLN

LEU

ARG

HIS

TYR

SER

ARG

PHE

VAL

ALA

11

LYS

GLU

ILE

ALA

ASP

SER

LYS

Samples:

sample_1: Tim12, [U-13C; U-15N], 0.2 mM; MES 20 mM; sodium chloride 50 mM; Tim9 0.2 mM; Tim10 0.2 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

ANALYSIS - chemical shift assignment

NMRPipe - processing

NMR spectrometers:

Bruker AVANCE III 600 MHz
Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks