BMRB Entry 50853

Title:
Heme chemical shifts assignment of the PpcBF6L from Geobacter sulfurreducens
Deposition date:
2021-03-26
Original release date:
2021-07-14
Authors:
Silva, Marta; Portela, Pilar; Salgueiro, Carlos
Citation:

Citation: Silva, Marta; Portela, Pilar; Salgueiro, Carlos. "Rational design of electron/proton transfer mechanisms in the exoelectrogenic bacteria Geobacter sulfurreducens"  Biochem. J. 478, 2871-2887 (2021).
PubMed: 34190983

Assembly members:

Assembly members:
entity_1, polymer, 71 residues, Formula weight is not available
entity_HEM, non-polymer, 616.487 Da.

Natural source:

Natural source:   Common Name: Geobacter sulfurreducens   Taxonomy ID: 35554   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Geobacter sulfurreducens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pVA203_PpcBF6L

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts36

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PpcBF6L_Polypeptide1
2HEM 12
3HEM 22
4HEM 32

Entities:

Entity 1, PpcBF6L_Polypeptide 71 residues - Formula weight is not available

1   ALAASPTHRMETTHRLEUTHRALALYSASN
2   GLYASNVALTHRPHEASPHISLYSLYSHIS
3   GLNTHRILEVALPROASPCYSALAVALCYS
4   HISGLYLYSTHRPROGLYLYSILEGLUGLY
5   PHEGLYLYSGLUMETALAHISGLYLYSSER
6   CYSLYSGLYCYSHISGLUGLUMETLYSLYS
7   GLYPROTHRLYSCYSGLYGLUCYSHISLYS
8   LYS

Entity 2, HEM 1 - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: Cytochrome PpcBF6L from Geobacter sulfurreducens 1 mM; sodium phosphate 80 mM; sodium chloride 250 mM; D2O, [U-2H], 100%

sample_conditions_1: ionic strength: 250 mM; pH: 8.0; pressure: 1 atm; temperature: 289.2 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN - processing

SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz