Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50853
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Citation: Silva, Marta; Portela, Pilar; Salgueiro, Carlos. "Rational design of electron/proton transfer mechanisms in the exoelectrogenic bacteria Geobacter sulfurreducens" Biochem. J. 478, 2871-2887 (2021).
PubMed: 34190983
Assembly members:
entity_1, polymer, 71 residues, Formula weight is not available
entity_HEM, non-polymer, 616.487 Da.
Natural source: Common Name: Geobacter sulfurreducens Taxonomy ID: 35554 Superkingdom: Bacteria Kingdom: not available Genus/species: Geobacter sulfurreducens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pVA203_PpcBF6L
Entity Sequences (FASTA):
entity_1: ADTMTLTAKNGNVTFDHKKH
QTIVPDCAVCHGKTPGKIEG
FGKEMAHGKSCKGCHEEMKK
GPTKCGECHKK
Data type | Count |
1H chemical shifts | 36 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | PpcBF6L_Polypeptide | 1 |
2 | HEM 1 | 2 |
3 | HEM 2 | 2 |
4 | HEM 3 | 2 |
Entity 1, PpcBF6L_Polypeptide 71 residues - Formula weight is not available
1 | ALA | ASP | THR | MET | THR | LEU | THR | ALA | LYS | ASN | ||||
2 | GLY | ASN | VAL | THR | PHE | ASP | HIS | LYS | LYS | HIS | ||||
3 | GLN | THR | ILE | VAL | PRO | ASP | CYS | ALA | VAL | CYS | ||||
4 | HIS | GLY | LYS | THR | PRO | GLY | LYS | ILE | GLU | GLY | ||||
5 | PHE | GLY | LYS | GLU | MET | ALA | HIS | GLY | LYS | SER | ||||
6 | CYS | LYS | GLY | CYS | HIS | GLU | GLU | MET | LYS | LYS | ||||
7 | GLY | PRO | THR | LYS | CYS | GLY | GLU | CYS | HIS | LYS | ||||
8 | LYS |
Entity 2, HEM 1 - C34 H32 Fe N4 O4 - 616.487 Da.
1 | HEM |
sample_1: Cytochrome PpcBF6L from Geobacter sulfurreducens 1 mM; sodium phosphate 80 mM; sodium chloride 250 mM; D2O, [U-2H], 100%
sample_conditions_1: ionic strength: 250 mM; pH: 8.0; pressure: 1 atm; temperature: 289.2 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
TOPSPIN - processing
SPARKY - chemical shift assignment