BMRB Entry 5085

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5085
MolProbity Validation Chart

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Title:
1H, 13C, and 15N Chemical Shift Assignments for the Complex of the C-terminal Domain of Human Poly(A)-binding Protein and C-terminal Fragment of Paip2
Deposition date:
2001-07-18
Original release date:
2003-12-08
Authors:
Kozlov, G.; Siddiqui, N.; Coillet-Matillon, S.; Ekiel, I.; Gehring, K.
Citation:

Citation: Kozlov, G.; Trempe, J.; Khaleghpour, K.; Kahvejian, A.; Ekiel, I.; Gehring, K.. "Structure and function of the C-terminal PABC domain of human poly(A)-binding protein"  Proc. Natl. Acad. Sci. U. S. A. 98, 4409-4413 (2001).
PubMed: 11287632

Assembly members:

Assembly members:
C-terminal domain of PABP, polymer, 98 residues, 10337 Da.
Paip2 (106-127), polymer, 22 residues, 2160 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-terminal domain of PABP: GPLGSPLTASMLASAPPQEQ KQMLGERLFPLIQAMHPTLA GKITGMLLEIDNSELLHMLE SPESLRSKVDEAVAVLQAHQ AKEAAQKAVNSATGVPTV
Paip2 (106-127): VVKSNLNPNAKEFVPGVKYG NI

Data sets:
Data typeCount
1H chemical shifts707
13C chemical shifts182
15N chemical shifts107
coupling constants101

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1polyadenylate-binding protein 1 (544-636)1
2polyadenylate-binding protein-interacting protein 2 (106-127)2

Entities:

Entity 1, polyadenylate-binding protein 1 (544-636) 98 residues - 10337 Da.

1   GLYPROLEUGLYSERPROLEUTHRALASER
2   METLEUALASERALAPROPROGLNGLUGLN
3   LYSGLNMETLEUGLYGLUARGLEUPHEPRO
4   LEUILEGLNALAMETHISPROTHRLEUALA
5   GLYLYSILETHRGLYMETLEULEUGLUILE
6   ASPASNSERGLULEULEUHISMETLEUGLU
7   SERPROGLUSERLEUARGSERLYSVALASP
8   GLUALAVALALAVALLEUGLNALAHISGLN
9   ALALYSGLUALAALAGLNLYSALAVALASN
10   SERALATHRGLYVALPROTHRVAL

Entity 2, polyadenylate-binding protein-interacting protein 2 (106-127) 22 residues - 2160 Da.

1   VALVALLYSSERASNLEUASNPROASNALA
2   LYSGLUPHEVALPROGLYVALLYSTYRGLY
3   ASNILE

Samples:

sample_1: C-terminal domain of PABP, [U-15N], 3 mM; Paip2 (106-127) 4 mM; phosphate buffer 50 mM; NaCl 0.1 M; NaN3 1 mM; H2O 90%; D2O 10%

sample_2: C-terminal domain of PABP, [U-15N; U-13C], 3 mM; Paip2 (106-127) 4 mM; phosphate buffer 50 mM; NaCl 0.1 M; NaN3 1 mM; D2O 100%

sample_3: C-terminal domain of PABP 3 mM; Paip2 (106-127), [U-15N], 2.5 mM; phosphate buffer 50 mM; NaCl 0.1 M; NaN3 1 mM; H2O 90%; D2O 10%

sample_4: C-terminal domain of PABP 3 mM; Paip2 (106-127), [U-15N; U-13C], 2 mM; phosphate buffer 50 mM; NaCl 0.1 M; NaN3 1 mM; D2O 100%

sample_5: C-terminal domain of PABP 3 mM; Paip2 (106-127) 3 mM; phosphate buffer 50 mM; NaCl 0.1 M; NaN3 1 mM; H2O 90%; D2O 10%

sample_6: C-terminal domain of PABP 3 mM; Paip2 (106-127) 3 mM; phosphate buffer 50 mM; NaCl 0.1 M; NaN3 1 mM; D2O 100%

sample_cond_1: pH: 6.3; temperature: 303 K; ionic strength: 0.1 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablesample_cond_1
3D 13C-separated NOESYnot availablenot availablesample_cond_1
2D NOESYnot availablenot availablesample_cond_1

Software:

XWINNMR v2.1 - collection

GIFA v4.31 - processing

XEASY v1.3.13 - data analysis

ARIA v0.9 - structure solution

CNS v0.9 - refinement

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Varian UnityPlus 800 MHz

Related Database Links:

PDB
DBJ BAB24053 BAB26578 BAE27082 BAE29989 BAE32597 BAE32597 BAE29989 BAE27082 BAB26578 BAB24053
EMBL CAB66575 CAG38520 CAH93070 CAL38574 CAL38574 CAH93070 CAG38520 CAB66575
GB AAH01716 AAH10190 AAH39337 AAH48106 AAH62718
REF NP_001014170 NP_001028284 NP_001029808 NP_001126818 NP_001244181 NP_080696 NP_057564 NP_001029808 NP_001028284 NP_001014170
SP Q3ZC67 Q5R596 Q6AXZ0 Q9BPZ3 Q9D6V8
TPG DAA27337
SWISS-PROT Q9D6V8 Q9BPZ3 Q6AXZ0 Q5R596 Q3ZC67
GenBank AAH62718 AAH48106 AAH39337 AAH10190 AAH01716
AlphaFold Q3ZC67 Q5R596 Q6AXZ0 Q9BPZ3 Q9D6V8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks