BMRB Entry 50848

Name: methylated ZEBRA-responsive element 2 DNA
Published: 2021-11-24

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50848

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

methylated ZEBRA-responsive element 2 DNA

Deposition date:

2021-03-25

Original release date:

2021-11-24

Authors:

Gunther, Johannes

Citation:

Citation: Bernaudat, Florent; Gustems, Montse; Gunther, Johannes; Oliva, Mizar; Buschle, Alexander; Gobel, Christine; Pagniez, Priscilla; Lupo, Julien; Signor, Luca; Muller, Christoph; Morand, Patrice; Sattler, Michael; Hammerschmidt, Wolfgang; Petosa, Carlo. "Structural basis of DNA methylation-dependent site selectivity of the Epstein-Barr virus lytic switch protein ZEBRA/Zta/BZLF1" Nucleic Acids Res. 50, 490-511 (2022).
PubMed: 34893887

Assembly members:

Assembly members:
entity_1, polymer, 18 residues, Formula weight is not available
entity_2, polymer, 18 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human gammaherpesvirus 4 Taxonomy ID: 10376 Superkingdom: Viruses Kingdom: not available Genus/species: Lymphocryptovirus Epstein Barr Virus

Experimental source:

Experimental source: Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GCTTATGAGXGATTTTAT
entity_2: CGAATACTCGXTAAAATA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	42

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Chain A	1
2	Chain B	2

Entities:

Entity 1, Chain A 18 residues - Formula weight is not available

1

DG

DC

DT

DA

DT

DG

DA

DG

5MC

2

DG

DA

DT

DA

DT

Entity 2, Chain B 18 residues - Formula weight is not available

1

DC

DG

DA

DT

DA

DC

DT

DC

DG

2

5MC

DT

DA

DT

DA

Samples:

sample_1: methylated ZEBRA-responsive element 2 DNA (ZRE2) 400 uM; sodium phosphate 50 mM; sodium chloride 100 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v2.4.2 - chemical shift assignment and data analysis

TOPSPIN v3.5 pl6 - processing

NMR spectrometers:

Bruker AVANCE III HD 950 MHz