BMRB Entry 50845

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50845

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Title:
Prefoldin Pyrococcus Horikoshii
Deposition date:
2021-03-24
Original release date:
2021-05-27
Authors:
Toerner, Ricarda; Boisbouvier, Jerome
Citation:

Citation: Toerner, Ricarda; Henot, Faustine; Awad, Rida; Macek, Pavel; Gans, Pierre; Boisbouvier, Jerome. "Backbone and methyl resonances assignment of the 87 kDa prefoldin from Pyrococcus horikoshii"  Biomol. NMR Assignments 15, 351-360 (2021).
PubMed: 33988824

Assembly members:

Assembly members:
entity_1, polymer, 117 residues, Formula weight is not available
entity_2, polymer, 148 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pyrococcus hirokoshii   Taxonomy ID: 53953   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus hirokoshii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET23c

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MQNIPPQVQAMLGQLDTYQQ QLQLVIQQKQKVQADLNEAK KALEEIETLPDDAQIYKTVG TLIVKTTKEKAVQELKEKIE TLEVRLNALNRQEQKINEKV KELTQKIQAALRPPTAG
entity_2: MAQNNKELEKLAYEYQVLQA QAQILAQNLELLNLAKAEVQ TVRETLENLKKIEEEKPEIL VPIGAGSFLKGVIVDKNNAI VSVGSGYAVERSIDEAIGFL EKRLKEYDEAIKKTQGALAE LEKRIGEVARKAQEVQQKQS MTSFKVKK

Data typeCount
13C chemical shifts753
15N chemical shifts216
1H chemical shifts606

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PhPFD beta1
2PhPFD alpha2

Entities:

Entity 1, PhPFD beta 117 residues - Formula weight is not available

1   METGLNASNILEPROPROGLNVALGLNALA
2   METLEUGLYGLNLEUASPTHRTYRGLNGLN
3   GLNLEUGLNLEUVALILEGLNGLNLYSGLN
4   LYSVALGLNALAASPLEUASNGLUALALYS
5   LYSALALEUGLUGLUILEGLUTHRLEUPRO
6   ASPASPALAGLNILETYRLYSTHRVALGLY
7   THRLEUILEVALLYSTHRTHRLYSGLULYS
8   ALAVALGLNGLULEULYSGLULYSILEGLU
9   THRLEUGLUVALARGLEUASNALALEUASN
10   ARGGLNGLUGLNLYSILEASNGLULYSVAL
11   LYSGLULEUTHRGLNLYSILEGLNALAALA
12   LEUARGPROPROTHRALAGLY

Entity 2, PhPFD alpha 148 residues - Formula weight is not available

1   METALAGLNASNASNLYSGLULEUGLULYS
2   LEUALATYRGLUTYRGLNVALLEUGLNALA
3   GLNALAGLNILELEUALAGLNASNLEUGLU
4   LEULEUASNLEUALALYSALAGLUVALGLN
5   THRVALARGGLUTHRLEUGLUASNLEULYS
6   LYSILEGLUGLUGLULYSPROGLUILELEU
7   VALPROILEGLYALAGLYSERPHELEULYS
8   GLYVALILEVALASPLYSASNASNALAILE
9   VALSERVALGLYSERGLYTYRALAVALGLU
10   ARGSERILEASPGLUALAILEGLYPHELEU
11   GLULYSARGLEULYSGLUTYRASPGLUALA
12   ILELYSLYSTHRGLNGLYALALEUALAGLU
13   LEUGLULYSARGILEGLYGLUVALALAARG
14   LYSALAGLNGLUVALGLNGLNLYSGLNSER
15   METTHRSERPHELYSVALLYSLYS

Samples:

sample_1: PhPFD beta, [U-13C; U-15N; U-2H], 0.8 uM; PhPFD alpha, [U-2H], 0.4 uM

sample_2: PhPFD beta, [U-12C; U-15N; U-2H; 1HD-Ile;2HD-Leu;2HG-Val;HB-Ala;2HG-Thr], 0.8 uM; PhPFD alpha, [U-2H], 0.4 uM

sample_3: PhPFD beta, [U-13C; U-15N; U-2H; 1HD-Ile;2HD-Leu;2HG-Val], 0.8 uM; PhPFD alpha, [U-2H], 0.4 uM

sample_4: PhPFD beta, [U-12C; U-14N; U-2H;2HD-Leu;2HG-Val], 0.3 uM; PhPFD alpha, [U-2H], 0.15 uM

sample_5: PhPFD alpha, [U-13C; U-15N; U-2H; 1HD-Ile;1HD-Leu;1HG-Val], 0.8 uM; PhPFD beta, [U-2H], 0.4 uM

sample_6: PhPFD alpha, [U-12C; U-15N; U-2H; 1HD-Ile;2HD-Leu;2HG-Val;HB-Ala;2HG-Thr], 0.8 uM; PhPFD beta, [U-2H], 0.4 uM

sample_7: PhPFD beta, [U-2H], 0.8 uM; PhPFD alpha, [U-13C; U-15N; U-2H], 0.4 uM

sample_conditions_1: ionic strength: 0.07 M; pH: 8.5; pressure: 1 atm; temperature: 343.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
2D 1H-15N TROSYsample_2isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_2isotropicsample_conditions_1
2D 1H-13C HMQCsample_3isotropicsample_conditions_1
2D 1H-13C HMQCsample_4isotropicsample_conditions_1
3D 1H-13C NOESYsample_3isotropicsample_conditions_1
3D 1H-13C NOESYsample_4isotropicsample_conditions_1
3D HCCH-COSYsample_5isotropicsample_conditions_1
3D HC(C)CH-COSYsample_5isotropicsample_conditions_1
3D HC(CC)CH-COSYsample_5isotropicsample_conditions_1
3D HCCH-COSYsample_6isotropicsample_conditions_1
3D HC(C)CH-COSYsample_6isotropicsample_conditions_1
3D HC(CC)CH-COSYsample_6isotropicsample_conditions_1
2D 1H-13C HMQCsample_7isotropicsample_conditions_1
2D 1H-13C HMQCsample_7isotropicsample_conditions_1
2D 1H-13C HMQCsample_7isotropicsample_conditions_1
2D 1H-13C HMQCsample_7isotropicsample_conditions_1
2D 1H-13C HMQCsample_7isotropicsample_conditions_1

Software:

ANALYSIS - chemical shift assignment

TOPSPIN - collection

NMRPipe - processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 850 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks