BMRB Entry 50837

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50837

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Title:
1H, 13C, and 15N resonance assignments of Mg2+-Calmodulin bound to BP2 derived from the STRA6 vitamin A transporter
Deposition date:
2021-03-16
Original release date:
2021-10-22
Authors:
Young, Brianna; Varney, Kristen; Cook, Mary; Weber, David
Citation:

Citation: Young, Brianna; Varney, Kristen; Wilder, Paul; Costabile, Brianna; Pozharski, Edwin; Cook, Mary; Godoy-Ruiz, Raquel; Clarke, Oliver; Mancia, Filippo; Weber, David. "Physiologically Relevant Free Ca 2+ Ion Concentrations Regulate STRA6-Calmodulin Complex Formation via the BP2 Region of STRA6"  J. Mol. Biol. 433, 167272-167272 (2021).
PubMed: 34592217

Assembly members:

Assembly members:
entity_1, polymer, 148 residues, Formula weight is not available
entity_MG, non-polymer, 24.305 Da.
entity_BP2, polymer, 27 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
entity_BP2: VSNAKRARAHWQLLYTLVNN PSLVGSR

Data sets:
Data typeCount
13C chemical shifts403
15N chemical shifts131
1H chemical shifts131

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Calmodulin1
2Mg2+2
3BP23

Entities:

Entity 1, Calmodulin 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, Mg2+ - Mg - 24.305 Da.

1   MG

Entity 3, BP2 27 residues - Formula weight is not available

1   VALSERASNALALYSARGALAARGALAHIS
2   TRPGLNLEULEUTYRTHRLEUVALASNASN
3   PROSERLEUVALGLYSERARG

Samples:

sample_1: Calmodulin, [U-13C; U-15N], 0.25 mM; STRA6 BP2 1.3 mM; Magnesium ion 20 mM; HEPES 20 mM; sodium chloride 50 mM; EGTA 10 mM; magnesium chloride 20 mM; TCEP 0.5 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCACONHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1

Software:

CcpNMR - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz MHz
  • Bruker AVANCE III 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks