BMRB Entry 5082
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5082
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Wang, Iren; Wu, Shih-Hsiung; Chang, Hsueh-Kai; Shieh, Ru-Chi; Yu, Hui-Ming; Chen, Chinpan. "Solution Structure of a K(+)-Channel Blocker from the Scorpion Tityus cambridgei" Protein Sci. 11, 390-400 (2002).
PubMed: 11790849
Assembly members:
Tityustoxin alpha-KTx, polymer, 23 residues, 2448 Da.
Natural source: Common Name: Tityustoxin alpha-KTx Taxonomy ID: not available Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Tityus cambridgei
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Tityustoxin alpha-KTx: ACGSCRKKCKGSGKCINGRC
KCY
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 155 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tc1 | 1 |
Entities:
Entity 1, Tc1 23 residues - 2448 Da.
| 1 | ALA | CYS | GLY | SER | CYS | ARG | LYS | LYS | CYS | LYS | ||||
| 2 | GLY | SER | GLY | LYS | CYS | ILE | ASN | GLY | ARG | CYS | ||||
| 3 | LYS | CYS | TYR |
Samples:
sample_1: Tityustoxin alpha-KTx1.5 – 2.0 mM
Ex-cond_1: pH: 3.0; temperature: 275 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H NOESY | not available | not available | not available |
| 1H-1H TOCSY | not available | not available | not available |
| 1H-1H COSY | not available | not available | not available |
Software:
xwinnmr v2.5 - data processing
NMR spectrometers:
- Bruker AMX 500 MHz
- Bruker Avance 600 MHz